布鲁氏菌MLVA-15分型方法的建立  

Establishment of a New Genotyping Method of MLVA-15 on Brucella

作　　者：王勋[1] 米吉提.莫合他尔汗 孙明军[3] 狄栋栋[3] 许芳[1] 贾舒安 颜成旭 李金平 范伟兴[3] 曾巧英[1] WANG Xun;MI Ji-ti;SUN Ming-jun;DI Dong-dong;XU Fang;JIA Shu-an;YAN Cheng-xu;LI Jin-ping;FAN Wei-xing;ZENG Qiao-ying(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Xinjiang Center of Animal Disease Control,Urumqi 830000,China;Laboratory of Zoonoses,China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]新疆动物卫生监督所,乌鲁木齐830000 [3]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国兽药杂志》2018年第8期12-18,共7页Chinese Journal of Veterinary Drug

基　　金：甘肃农业大学伏羲杰出人才项目;现代农业(奶牛)产业技术体系建设项目专项经费(CARS-36)

摘　　要：为提高对我国布鲁氏菌流行菌株的鉴别分型能力,利用布鲁氏菌可变数目串联重复序列(VNTR)的多态性,建立了新的布鲁氏菌分型方法——MLVA-15。与传统的布鲁氏菌MLVA-16和BruMLSA21分型方法相比,新的MLVA-15分型方法对我国羊种布鲁氏菌流行菌株的区分能力有着显著的提高,能够满足布病流行病学调查中对病原追溯的需求。尝试利用不同VNTR位点组合来提高对布鲁氏菌的鉴别能力,为单一种型布鲁氏菌流行区域的布病病原学调查提供了新思路。In order to improve the ability of identifying and discriminating Brucella strains epidemic in China,variable number tandem repeat（VNTR） polymorphism was valued and a new Brucella typing method of MLVA-15 was established.Compared to the traditional Brucella MLVA-16 and BruMLSA21 typing methods,the new MLVA-15 method has significantly improved the ability in differentiating Brucella melitensis strains currently circulating in China.It can also meet the requirement of infection source tracing back in the epidemiological investigation of brucellosis.Using different combinations of VNTR loci to improve the ability of identifing Brucella strains provides a means for the etiological investigation in regions where a single species of Brucella prevails.

关 键 词：布鲁氏菌 VNTR MLVA BruMLSA 

分 类 号：S852.61[农业科学—基础兽医学]