CD74低表达对人胰腺癌细胞增殖、迁移和侵袭的影响  

Effects of low expression of CD74 on proliferation, migration and invasion of human pancreatic cancer cells

作　　者：黄耀星[1] 贾林[1] 王康玮 余丹纯 孙小娟 江舒曼[1] Huang Yaoxing;Jia Lin;Wang Kangwei;Yu Danchun;Sun Xiaojuan;Jiang Shuman(Department of Gastroenterology,Nansha Hospital,Guangzhou First Municipal People＇ s Hospital Affiliated to Guangzhou Medical University,Guangzhou,Guandong 510180,China)

机构地区：[1]广州医科大学附属广州市第一人民医院广州市第一人民医院南沙医院消化内科,510180 [2]广州医科大学附属广州市第一人民医院广州市第一人民医院南沙医院病理科,510180

出　　处：《中华生物医学工程杂志》2017年第5期363-368,共6页Chinese Journal of Biomedical Engineering

基　　金：广东省医学科研基金（A2014549）

摘　　要：目的探讨干扰CD74表达对人胰腺癌细胞株Capan-2细胞生物学行为的影响。方法设计CD74．短发夹RNA（CD74-shRNA）干扰序列构建CD74干扰质粒转染Capan-2细胞（CD74-shRNA组），以转染pSUPER空载质粒为对照，采用MTr法检测细胞增殖活力，划痕实验和Transwell实验检测细胞迁移和侵袭，实时定量PCR和免疫印迹法检测CD74mRNA和蛋白表达，实时定量PCR检测细胞基质金属蛋白酶2（MMP-2）和MMP-9mRNA表达。结果细胞增殖活力检测和划痕实验显示，干扰CD74表达可降低Capan-2细胞的增殖活力、抑制细胞迁移（均P〈0．05）。Transwell实验显示，培养48h后CD74-shRNA组和对照组的穿膜细胞数分别为（102．5±9．03）个/孔、（139．1±9．89）个／孔，CD74-shRNA组显著低于对照组（P〈0．05）。相对于对照组，CD74-shRNA组细胞CD74mRNA和蛋白表达抑制率分别为61％、90％，MMP-2和MMP-9mRNA表达抑制率分别为84％、85％。结论CD74低表达可抑制Capan-2细胞增殖、迁移和侵袭，CD74可能作为胰腺癌治疗的新靶点。Objective To investigate the effect of interfering CD74 expression on the biological behavior of human pancreatic cancer cell line Capan-2. Methods The CD74-short hairpin RNA （CD74- shRNA） interference sequence was designed to construct CD74 interference plasmid transfected Capan- 2 cells （group CD74-shRNA）. The transfected pSUPER empty vectors were included as the controls. MTT assay was used to determine the cell proliferation activity. The scratch assay and Transwell assay were used to determine the cell migration and invasion. The real-time quantitative PCR and Western blotting were used to examine CD74 mRNA and protein expression. The real- time quantitative PCR was used to examine the mRNA expression of matrix metalloproteinase 2 （MMP-2） and MMP-9. Results The cell proliferation activity test and scratch test showed that interfering CD74 expression could decrease Capan- 2 cell proliferation and inhibit cell migration （all P〈0.05）. The Transwell assay showed that the number of transmemhrane cells in the CD74-shRNA group and the control group after culturing for 48 h were （102.5± 9.03）/well and （139.1 ± 9.89）/well, respectively, and the number in the CD74- shRNA group was significantly lower than that in the control group （P〈0.05）. Compared with the control group, the inhibition rates of CD74 mRNA and protein expression in the CD74-shRNA group were 61% and 90%, respectively, and the inhibition rates of MMP-2 and MMP-9 mRNA expression in the CD74-shRNA group were 84% and 85%, respectively. Conclusion Tile low expression of CD74 can inhibit the proliferation, migration and invasion of Capan-2 cells. CD74 may serve as a new target for the treatment of pancreatic cancer.

关 键 词：胰腺癌 抗原 CD74 细胞增殖 细胞迁移 侵袭 Capan-2细胞系 

分 类 号：R7[医药卫生—临床医学] R4