虫草素对人宫颈癌Hela细胞株增殖及凋亡的影响  

Effect of cordycepin on proliferation and apoptosis of Hela cells

作　　者：陈玉芬 洪龙年 陈祝明 张颖 CHEN Yu-fen;HONG Long-nian;CHEN Zhu-ming;ZHANG Ying(Department of Obstetrics and Gynecology;Department of Orthopedic Surgery;Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China)

机构地区：[1]广东医科大学附属医院妇产科,广东湛江524001 [2]广东医科大学附属医院骨科中心,广东湛江524001

出　　处：《广东医科大学学报》2018年第3期247-250,255,共5页Journal of Guangdong Medical University

摘　　要：目的观察不同浓度虫草素对人宫颈癌Hela细胞增殖与凋亡的影响。方法不同浓度虫草素处理Hela细胞24 h,倒置显微镜观察细胞形态变化,CCK-8法检测细胞增殖活性,流式细胞术检测细胞凋亡,Western-blot检测凋亡相关蛋白。结果 Hela细胞增殖活性随虫草素浓度增加而降低(P<0.05),虫草素引起Hela细胞早期凋亡(P<0.05),降低Bcl-2蛋白、增加Bax蛋白表达,诱导Cleaved-PARP裂解。结论虫草素通过下调Hela细胞中Bcl-2并上调Bax表达,促进早期凋亡。Objective To observe the effect of different concentrations of cordycepin on the proliferation and apoptosis of human cervical carcinoma Hela cells. Methods Hela cells were treated with different concentrations of cordycepin for 24 h, and their morphology, proliferation, apoptosis, and apoptosis-related protein levels were detected inverted microscopy, CCK-8, flow cytometry, and Western blot, respectively. Results The proliferative activity of Hela cells was decreased as incremental concentrations of cordycepin（P〈0.05）. Cordycepin induced the early apoptosis（P〈0.05） and Bax and cleavedPARP expression, but reduced Bcl-2 expression in Hela cells. Conclusion Cordycepin can promote early apoptosis via downregulating Bcl-2 and upregulating Bax expression in Hela cells.

关 键 词：宫颈癌 HELA细胞 虫草素 增殖 凋亡 

分 类 号：R711[医药卫生—妇产科学]