人参皂苷Rg1对新生鼠缺氧缺血性脑损伤海马神经元凋亡及学习记忆能力的影响  

Effects of ginseng Rg1 on hippocampal neuronal apoptosis and learning ability of neonatal rats with hypoxic-ischemic brain damage

作　　者：雷勋明 LEI Xunming(Children＇s Hospital of Chengdu Medical College,Chengdu 610041,China)

机构地区：[1]成都医学院附属妇女儿童医院儿童健康中心,成都610041

出　　处：《中国中西医结合儿科学》2018年第4期277-279,共3页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：湖北省教育厅基金资助(B2014049)

摘　　要：目的探讨人参皂苷Rg1对新生鼠缺氧缺血性脑损伤(HIBD)海马神经元凋亡及学习记忆能力的影响。方法选择15日龄SD乳鼠60只,随机分为HIBD组、人参皂苷Rg1低剂量组和高剂量组各20只。人参皂苷Rg1高、低剂量组术后每天经腹腔注射人参皂苷Rg1 20、10μg/g,HIBD组注射等量生理盐水,各组大鼠均连续注射25d。采用TUNEL法检测海马CA1区神经元凋亡情况,对各组大鼠进行学习能力、记忆力测试,计算其正确反应率。结果 HIBD组海马CA1区TUNEL阳性细胞数高于注射人参皂苷Rg1高、低剂量组,人参皂苷Rg1低剂量组海马CA1区TUNEL阳性细胞数高于高剂量组,差异有统计学意义(P<0.05)。HIBD组测试学习能力和记忆力正确反应率低于注射人参皂苷Rg1高、低剂量组,且人参皂苷Rg1低剂量组学习能力及记忆力测试正确反应率低于高剂量组,差异有统计学意义(P<0.05)。结论人参皂苷Rg1能减少新生鼠HIBD海马神经元凋亡,有效地改善HIBD新生鼠的学习能力和记忆力。A total of 60 15-day SD rats were chosen and randomly divided into 3 groups： HIBD group, ginseng Rg1 low-dose group and high-dose group, 20 rats in each group. After surgery, ginseng Rg1 was peritoneally injected at 20 μg/g and 10 μg/g every day in the high-dose and low-dose group, while normal saline was injected in HIBD group. The injection continued for 25 days. TUNEL method was used to detect the neuronal apoptosis in hippocampal CA1 area, the learning ability and memory of the rats in each group were tested, and the correct response rate was calculated.Results The number of TUNEL positive cells in hippocampus CA1 area of low-dose group and high-dose group was lower than that of HIBD group, and the number of TUNEL positive cells in hippocampus CA1 area of high-dose group was lower than that in low-dose group（ P 〈0.05）. The correct response rate of learning ability and memory test in low-dose group and high-dose group was higher than that in HIBD group. The correct response rate of learning ability and memory test in high-dose group was higher than that in low-dose group（ P 〈0.05）.Conclusion Ginseng Rg1 can reduce the apoptosis of hippocampal neurons in neonatal rats with hypoxic-ischemic brain damage, and effectively improve the learning ability and memory of these rats.

关 键 词：缺氧缺血性脑损伤 细胞凋亡 人参皂苷RG1 大鼠 

分 类 号：R3[医药卫生—基础医学]