健脾益肠散对溃疡性结肠炎大鼠CD40表达的影响  

Effect of Jianpi Yichang Powder on CD40 Expresson in UC Rats

作　　者：刘杰民[1] 张瑜[2] 古娟 钟日君 王敏[2] 赵琦[2] 蔺晓源[3,4] LIU Jiemin;ZHANG Yu;GU Juan;ZHONG Rijun;WANG Min;ZHAO Qi;LIN Xiaoyuan(Guizhou Provincial People＇s Hospital,Guiyang 550002,China;Guiyang University of Chinese Medicine,Guiyang 550002,China;Hunan University of Chinese Medicine,Changsha 410208,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]贵州省人民医院,贵州贵阳550002 [2]贵阳中医学院,贵州贵阳550002 [3]湖南中医药大学,湖南长沙410208 [4]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中医药信息》2018年第5期5-8,共4页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81560773)

摘　　要：目的:探讨健脾益肠散对溃疡性结肠炎(UC)大鼠CD40分子蛋白和基因表达的影响。方法:SD大鼠60只,随机分为空白组,模型组,健脾益肠散高、中、低剂量组和西药组(柳氮磺吡啶组);采用TNBS/乙醇法制备UC大鼠模型;灌胃相应药物21 d后,免疫组化、Western blot和RT-PCR法分别检测各组大鼠结肠组织中CD40的蛋白和mRNA表达。结果:模型组大鼠结肠组织CD40的蛋白和mRNA表达较空白组显著升高(P <0. 01)。各给药组均可不同程度地降低结肠CD40蛋白和mRNA的表达(P <0. 05或P <0. 01),且以健脾益肠散高剂量最为明显,而健脾益肠散中剂量与柳氮磺吡啶比较差异不显著(P> 0. 05)。结论:健脾益肠散对UC肠黏膜的免疫保护可能与降低CD40的表达有关。Objective： To explore the effect of Jianpi Yichang Powder（JP） on protein and mRNA expressions of CD40 molecule in rats with ulcerative colitis（ UC ）. Methods： 60 SD rats were randomly divided into the blank group, the model group, JP groups（low-dose, medium- dose, and high -dose） , and Sulfasalazine group. The UC rat models were established by TNBS/ethanol method. The protein and mRNA expressions of CD40 were detected by immunohistochemical method, Western blot and RT - PCR methods after 21 - day intervention. Results： The protein and mRNA expressions of CD40 were significantly increased in the model group compared to those in the blank group （P 〈 0.01 ） ; they were significantly decreased in all medication groups （P 〈 0.05, P 〈 0.01 ）, in which the high - dose JP group had the best effect; there was no significant difference between the medium - dose JP group and Sulfasalazine group （P 〉 0.05 ）. Conclusion ： The immune protection of JP on UC intestinal mucosa may be related to the decrease of CD40 expression.

关 键 词：健脾益肠散 溃疡性结肠炎 大鼠 CD40 

分 类 号：R285.5[医药卫生—中药学]