玉米叶形相关性状的Meta-QTL及候选基因分析  

Analysis of Meta-quantitative Trait Loci and Their Candidate Genes Related to Leaf Shape in Maize

作　　者：郭书磊[1,2] 张君 齐建双[1] 岳润清[1] 韩小花[1] 燕树锋[1] 卢彩霞[1] 傅晓雷[1] 陈娜娜 库丽霞[2] 铁双贵 Shulei Guo;Jun Zhang;Jianshuang Qi;Runqing Yue;Xiaohua Han;Shufeng Yan;Caixia Lu;Xiaolei Fu;Nana Chen;Lixia Ku;Shuanggui Tie(Henan Provincial Key Lab of Maize Biology,Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Collaborative Innovation Center of Henan Grain Crops,College of Agronomy,Henan Agriculture University,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院粮食作物研究所河南省玉米生物学重点实验室,郑州450002 [2]河南农业大学农学院河南粮食作物协同创新中心,郑州450002

出　　处：《植物学报》2018年第4期487-501,共15页Chinese Bulletin of Botany

基　　金：河南省现代农业产业技术体系专项(No.S2015-02-G02);河南省科技开放合作项目(No.172106000040);中国博士后科学基金(No.2017M612404);河南省博士后科研资助项目(No.1515)

摘　　要：叶长、叶宽、叶面积及叶夹角不仅影响玉米(Zea mays)光合效率,也是株型的重要构成因素。通过对620个叶形QTL进行整合,构建不同遗传背景下的叶形QTL整合图谱,利用元分析发掘出22个叶长、22个叶宽、12个叶面积以及17个叶夹角m QTL;进一步运用生物信息学手段,确定44个与叶片发育密切相关的候选基因。分析发现,仅有NAL7-like、YABBY6-like和GRF2等13个基因位于m QTL区间内,而玉米中已克隆的KNOTTED1、AN3/GIF1、rgd1/lbl1、mwp1及SRL2-like、HYL1-like和CYCB2;4-like等水稻(Oryza sativa)和拟南芥(Arabidopsis thaliana)叶形同源基因位于未被整合的QTL内;对44个候选基因在叶片长、宽、厚发育过程中基部-末端、中央-边缘、远轴-近轴的调控机理进行归纳分析,发现玉米中仅有少数几个候选基因被报道,揭示了叶形发育的部分分子机理。因此,对玉米叶形相关m QTL/QTL及基因进行全面深入的分析,不仅有助于增加对其遗传结构的了解,发掘更多候选基因,阐明叶形发育和形成的分子机制,还可为耐密理想株型的分子标记辅助选择提供依据。Leaf length, width, area, and angle are important components of plant architecture but also affect the efficiency of photosynthesis in maize. In this study, 620 quantitative trait loci（QTL） were used to construct an integrated map related to maize leaf shape; 22 maize QTL（m QTL） for leaf length, 22 for leaf width, 12 for leaf area and 17 for leaf angle were estimated by meta-analysis. Further bioinformatics analysis identified 44 candidate genes closely related to leaf shape within the m QTL region, with some unintegrated QTL. However, only 13 candidate genes, including NAL7-like, YABBY6-like, and GRF2, were located in the m QTL region. Most of the candidate genes, such as the cloned genes KNOTTED1, AN3/GIF1, rgd1/lbl1 and mwp1 in maize and SRL2-like, HYL1-like, and CYCB2;4-like in rice and Arabidopsis thaliana homologous genes were projected onto the interval of unintegrated QTL. The regulation mechanism of 44 candidate genes is summarized and analyzed in the development of leaf length, width and thickness, by proximal-distal, central-marginal and adaxial-abaxial. Only a few known genes revealed part of the molecular mechanism of leaf development in maize. Further research of the m QTL/QTL and related genes will create a global view of the genetic architecture of maize leaf shape, provide useful biological information for fine mapping QTL, and identify more candidate genes to clarify the molecular mechanism of leaf morphogenesis and provide a theoretical base for ideal plant-architecture improvement of maize marker-assisted breeding.

关 键 词：玉米 叶长 叶宽 叶面积 叶夹角 元分析 meta-QTL 候选基因 

分 类 号：S513[农业科学—作物学]