NF-κB对动脉粥样硬化斑块易损性作用的研究  

Role of NF-kappa B in atherosclerotic plaque vulnerability

作　　者：钟金鹏[1] 杨莉[2] 程彬 潘黎明[1] 李冠兰[1] 方玲[1] 吕云波[1] ZHONG Jin-peng;YANG Li;CHENG Bin;PAN Li-ming;LI Guan-lan;FANG Ling;LV Yun-bo(Department of Cardiology,Department of Neurology;the People ＂s Hospital of China Three Gorges University ＆ the First People ＂s Hospital of Yichang,Yichang 443000,Hubei,CHINA)

机构地区：[1]三峡大学人民医院&宜昌市第一人民医院心内科,湖北宜昌443000 [2]三峡大学人民医院&宜昌市第一人民医院神经内科,湖北宜昌443000

出　　处：《海南医学》2018年第16期2221-2225,共5页Hainan Medical Journal

基　　金：湖北省宜昌市科学技术基金(编号:A16-301-15)

摘　　要：目的研究动脉粥样硬化不稳定斑块中核因子κB(NF-κB)的表达情况,探讨NF-κB与斑块易损性之间的关系。方法健康兔24只,采用完全随机法分为对照组(Control组,8只,正常喂养)、稳定斑块组(AS组,8只,高脂喂养)、易损斑块组(VAP组,8只,高脂喂养+药物触发)。分别于喂养0周及16周时,各组动物抽取血液标本测定血脂、超敏C反应蛋白(hs-CRP);各组动物建模成功后,予以处死取主动脉标本,评价斑块稳定性,并采用免疫组化、Western-blot、RT-PCR技术检测斑块中NF-κB的表达,比较各组间的差异。结果组间区别喂养16周后,与Control组相比,AS组和VAP组兔的血脂、hs-CRP水平明显升高。当易损斑块经药物触发形成后,VAP组兔的校正斑块面积为(65.32±11.49)%,易损指数为(2.87±0.83),明显高于AS组的(40.58±6.88)%和(0.86±0.23),差异均有显著统计学意义(P<0.01)。在蛋白表达层面,免疫组化显示,斑块内NF-κB的表达在Control组、AS组、VAP组逐渐加强;Western-blot定量分析表明,VAP组兔的斑块内NF-κB表达水平为(2.01±0.69),明显高于AS组的(1.28±0.46)和Control组的(0.58±0.18),差异均有统计学意义(P<0.05)。在转录层面,RT-PCR检测亦提示VAP组兔的斑块内NF-κB转录水平为(13.2±4.2),显著高于AS组的(5.8±2.1)和Control组的(1.1±0.8),差异均有显著统计学意义(P<0.01)。结论与AS组和Control组比较,VAP组兔的动脉粥样硬化不稳定斑块中NF-κB的表达明显增加,提示NF-κB的上调表达与动脉斑块易损性密切相关。Objective To study the expression of nuclear factor kappa B（NF-κB） in unstable atherosclerotic plaques, and explore the relationship between NF-κB and plaque vulnerability. Methods A total of 24 healthy rabbits were divided into the control group（n=8, normal feeding）, atherosclerosis group（AS group, n=8, high fat feeding）, vulnerable plaque group（VAP group, n=8, high fat feeding ＋ drug trigger） according to random number table. Blood samples were collected for the examinations of lipid profiles and hs-CRP levels at 0 and 16 weeks of different feeding. Aortic specimens were obtained after successful modeling. The plaque stability was assessed and the expression of NF-κB was detected by immunohistochemistry, western-blot and RT-PCR. Differences were compared among the groups. Results After16 weeks of different feeding, the blood lipid profiles and hs-CRP levels in the AS and VAP groups were significantly higher than those in the control group. After the formation of vulnerable plaque by drug trigger, the corrected plaque area and vulnerability index were respectively（65.32 ± 11.49）% and（2.87 ± 0.83） in the VAP group, which were significantly higher than corresponding（40.58±6.88）% and（0.86±0.23） in the AS group（P0.01）. At the level of protein, the results of immunohistochemistry showed that the expression of NF-κ B gradually became deeper from the control group, AS group to VAP group. Western-blot showed that the level of NF-κB in the plaque of VAP group was（2.01±0.69）, which was higher than（1.28 ± 0.46） of the AS group and（0.58 ± 0.18） of the control group, and the differences were statistically significant（all P0.05）. At the level of m RNA, the results of RT-PCR showed that the transcription level of NF-κB in the plaque of the VAP group was（13.2±4.2）, which was higher than（5.8±2.1） in the AS group and（1.1±0.8） in the control group, and the differences were statistically significant（all P0.01）. Conclusion Compared to the AS group

关 键 词：动脉粥样硬化 核因子ΚB 易损斑块 稳定性 

分 类 号：R541.4[医药卫生—心血管疾病]