模拟失重对大鼠肝脏CYP1A2的影响研究  

Effect of Simulated Microgravity on Hepatic CYP1A2 in Rats

作　　者：李玉娟[1] 李盼盼 孙欣欣[1] 李勇枝[2] 王佳平[2] LI Yu-juan;LI Pan-pan;SUN Xin-xin;LI Yong-zhi;WANG Jia-ping(School of Life Science,Beijing Institute of Technology,Beijing 100081,China;China Astronaut Research and Training Centre,Beijing 100094,China)

机构地区：[1]北京理工大学生命学院,北京100081 [2]中国航天员科研训练中心,北京100094

出　　处：《北京理工大学学报》2018年第7期766-770,共5页Transactions of Beijing Institute of Technology

基　　金：国家自然科学基金资助项目(81573693);1226重大基金资助项目(AWS16J018);国家重大科学仪器设备开发专项基金资助项目(2012YQ40140)

摘　　要：为揭示失重条件下药物代谢酶变化规律,考察了不同模拟失重周期对鼠肝代谢酶CYP1A2的影响.将大鼠尾悬吊3,7,14,21d模拟失重效应,分别采用Western-blot、Q-PCR及HPLC-UV检测鼠肝微粒体中CYP1A2的蛋白表达、mRNA表达及活性变化.与对照组相比,模拟失重3d大鼠肝脏CYP1A2的蛋白表达量显著下降(p<0.05),7,14d显著上升(p<0.05),21d略有下降(p>0.05).在大鼠肝脏CYP1A2mRNA量及活性方面,模拟失重3,7,14d均显著上升(p<0.05),21d上升不明显(p>0.05).实验结果表明模拟失重14d鼠肝中的CYP1A2蛋白、基因表达及活性显著上升,但21d各指标有恢复到正常组的趋势.To provide basic research data for understanding the changes of metabolic enzymes under weightlessness, the effect of simulated microgravity on hepatic CYPIA2 in rats was investigated. After the rats are tail suspended for 3, 7, 14 and 21 days, the protein expression, mRNA expression and the CYP1A2 activity were detected by western-blot, Q-PCR and HPLC- UV methods. Compared with the control group, the expression of CYP1A2 protein decreases significantly （p〈0.05） in the 3 d tail suspended （ TS ） rats, but increases significantly （p〈 0.05 ） in the 7 and 14 d TS rats. The expression of protein decreases slightly （p〈 0.05 ） in the 21 d TS rats. The mRNA expression and activity of CYP1A2 increase significantly （p〈0.05） in 3, 7 and 14 d TS rats and increase slightly （p〈 0.05 ） in 21 d TS rats compared with the control group. The 14 d simulated group has the most significant effect on the expression and the activity of hepatic CYPIA2 in rats. All the indices in 21 d TS rats show the tendency to the control group level. This study provides basic research data for understanding changes of metabolic enzymes and drug safety of astronauts under the microgravity condition.

关 键 词：CYP1A2 模拟微重力效应 代谢酶 大鼠 

分 类 号：R917[医药卫生—药物分析学]