硫酸乙酰肝素蛋白聚糖在人乳头瘤病毒感染中的作用  

Research progress of heparan sulfate proteoglycan in the infection of human papillomavirus

作　　者：魏双萍 柳欣林 王大宁[2] 夏宁邵[1,2] 李少伟[1,2] WEI Shuang-ping;LIU Xin-lin;WANG Da-ning;XIA Ning-shao;LI Shao-wei(State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,School of Life Sciences,Xiamen University,Xiamen,Fujian 361102,China)

机构地区：[1]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心生命科学学院,福建厦门361102 [2]厦门大学分子疫苗学与分子诊断学国家重点实验室公共卫生学院,福建厦门361102

出　　处：《中国病毒病杂志》2018年第4期313-317,共5页Chinese Journal of Viral Diseases

基　　金：国家自然科学基金(31670935)

摘　　要：人乳头瘤病毒(human papillomavirus,HPV)是一类无包膜的小DNA双链病毒,其衣壳蛋白由主要衣壳蛋白L1和次要衣壳蛋白L2组成,高危型HPV的持续感染是诱发宫颈癌的主要原因。HPV吸附入胞伴随着由多种受体引起衣壳蛋白L1和L2的变构,其中衣壳蛋白L1与细胞表面硫酸乙酰肝素蛋白聚糖(heparan sulfate proteoglycans,HSPG)之间的多重相互作用是病毒入胞的关键。经与HSPG相互作用后,HPV衣壳蛋白L2暴露病毒与入胞受体结合位点,进而介导病毒内吞入胞。因此,阐述HPSG在HPV感染细胞中的作用有助于进一步阐明HPV感染机制及致病机制,为HPV治疗性疫苗的研究提供一定的理论基础。本文就HSPG在HPV感染细胞中的作用进行综述。Human papillomavirus（HPV）is a small,non-enveloped,double-stranded DNA virus consisting of the major（L1）and minor（L2）capsid proteins.High-risk HPV persistent infection is the primary cause of cervical cancer.HPV binding and entry are accompanied by several receptor-induced conformational changes of proteins L1 and L2.The multiple interactions of heparan sulfate proteoglycan（HSPG）on the cell-surface with L1 protein is essential for the virus entry.After binding to HSPG,the virion undergoes conformational changes,exposure the N-terminal L2 protein and interaction with several receptors that induce internalization of HPV.Therefore,studies on the roles of HPSG in HPV infection will help to elucidate the mechanism and pathogenesis of HPV infection,and provide insights for the research of HPV therapeutic vaccine.

关 键 词：人乳头瘤病毒 衣壳蛋白 宫颈癌 硫酸乙酰肝素蛋白聚糖(HSPG) 构象变化 受体 

分 类 号：R373.9[医药卫生—病原生物学]