正交设计法优选清毒消瘀颗粒水提液纯化工艺  

Optimization of Purification Process of Qingduxiaoyu Granule by Orthogonal Test

作　　者：王姗姗[1] 施崇精 刘小妹[1] 程中琴 何成诗[2] 宋英[2] Wang Shanshan;Shi Chongjing;Liu Xiaomei;Cheng Zhongqin;Song Ying(Chengdu University of Traditional Medicine（TCM）,Chengdu 610075,China;TeachingHospital of University of TCM,Chengdu 610072,China)

机构地区：[1]成都中医药大学,四川成都610075 [2]成都中医药大学附属医院,四川成都610072

出　　处：《亚太传统医药》2018年第8期28-33,共6页Asia-Pacific Traditional Medicine

基　　金：四川省科技计划项目(No.2014SZ0140)

摘　　要：目的:优选清毒消瘀方水提液的纯化工艺,减少有效成分的流失。方法:用HPLC法测定制剂中黄芩苷、芍药苷、落新妇苷的含量,首先进行纯化方法的单因素考察,然后用L25(54)正交试验考察离心转速、药液比及离心时间对该制剂水提液的影响,筛选其最佳纯化工艺条件。结果:最佳离心纯化工艺条件为:将清毒消瘀方水提液常压浓缩至药液比为1∶3,再进行离心纯化处理,离心转速为3 500r·min^(-1),离心时间为40min。结论:实验优选得到的清毒消瘀方水提液离心纯化工艺能够得到有效成分含量较高的纯化液,且方法稳定,适合工业化生产。Objective：To optimize the purificationprocess of Qing Du Xiao Yu granule,decrease the loss of active ingredients.Methods：HPLC was used to determine the content of baicalin,paeoniflorin,astilbin.Firstly,single factor studies of the purification method was carried out,and then,orthogonal test was adopted in the study.Taking the content of baicalin,paeoniflorin,astilbin and the dry extract yield as the indexes,orthogonal test was used to investigate impacts that centrifugal speed,liquid rate and time effecting on the purification process of Qing Du Xiao Yu granule.Finally,the optimum purificationprocess was screened.Results：The optimal purificationprocess was conducted by diluting the concentrate to liquid rate is 1∶3 with 3500 r·min^（-1） for 40 min.Conclusion：Conclusion：The method allows for higher purification of the active ingredient.And the extraction process is feasible and stable and suitable for industrial production.

关 键 词：清毒消瘀颗粒 正交试验 离心纯化工艺 

分 类 号：R284.2[医药卫生—中药学]