常温下两步反应法制备快速自固化的磷酸锶钙骨支架  

A two-step reaction to prepare a fast-hardening strontium-calcium phosphate scaffold in room temperature

作　　者：邝冠明 吴隽 刘飞宏 李晓 黄德民[2] 吕维加[2] Kuang Guanming;Wun Jun;Liu Feihong;Li Xiao;Wong Tak-man;Lu William(Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma,Department of Orthopaedics and Traumatology,The University of Hong Kong-Shenzhen Hospita;Department of Orthopaedics and Traumatology,The University of Hong Kon)

机构地区：[1]深圳骨科创伤修复新技术重点实验室,香港大学深圳医院骨科,518053 [2]香港大学矫形及创伤外科学系

出　　处：《中华创伤骨科杂志》2018年第8期717-723,共7页Chinese Journal of Orthopaedic Trauma

基　　金：广东省自然科学基金（2014A030313743）;深圳市科技计划项目（JCYJ20160429190045482）

摘　　要：目的 探讨一种常温下的两步反应法制备快速自固化的磷酸锶钙骨支架的可行性.方法 以粉状磷酸四钙（TTCP）、无水磷酸二钙（DCPA）、磷酸氢锶（DSPA）为原始反应物,按0、5％、10％、20％的锶/（锶＋钙）离子摩尔百分比混合,相应的组别分别命名为Sr-0组、Sr-5组、Sr-10组、Sr-20组.液相为含质量百分比为20％的柠檬酸和质量百分比为12％的聚乙烯吡咯烷酮混合液.在常温下,液相与粉剂按0.5 mL/g比例混合,制备骨支架.按标准方法测量骨支架的固化时间、压缩强度、分析固化后的结晶成分及其微观形貌,分析固化物浸提液的离子成分和pH值.结果 柠檬酸能加速骨支架的固化.调整柠檬酸的浓度和pH值能使骨支架固化时间控制在可接受的范围.锶离子不影响骨支架的强度和结晶成分,也不改变支架的内部微观形貌,固化产物的浸提液可见钙离子和锶离子析出,浸提液pH值无明显变化.结论 常温下的两步反应法是一种制备快速自固化磷酸锶钙骨支架的可行办法.Objective To evaluate a fast-hardening strontium-calcium phosphate scaffold using a two-step reaction in room temperature.Methods The original powder phase consisted of tetracalcium phosphate （TTCP）,dicalcium phosphate anhydrous （DCPA） and strontium hydrogen phosphate （DSPA）.The liquid phase consisted of 20wt% citric acid and 12wt% polyvinylpyrrolidone K-30.Groups were designed as Sr-0,Sr-5,Sr-10,and Sr-20,according to the molar ratios of Sr/（Sr ＋ Ca） being 0,5%,10%,and 20% in the powder phase,respectively.The ratio 0.5 mL/g in the liquid to solid phase was used for mixing.After the scaffold was prepared,its setting time,compression strength,phase composition and structural morphology,ions release and pH value of the substrates were analyzed.Results Addition of citric acid accelerated the setting reaction.Acceptable setting time was achieved by optimizing composition of the original powder and liquid phases.On the other hand,the compression strength,phase composition and structural morphology were not affected by addition of strontium.In addition,calcium and strontium ions were detected in the substrates,without any significant change in the pH value.Conclusion This two-step reaction can be used to prepare a fast hardening strontium-calcium phosphate scaffold in room temperature.

关 键 词：锶 磷酸钙类 羟基磷灰石类 固化时间 

分 类 号：R318.08[医药卫生—生物医学工程] R68[医药卫生—基础医学]