Assessment of the cytotoxic and mutagenic potential of the Jialu River and adjacent groundwater using human-hamster hybrid cells  

作　　者：Xiaofei Wang Guoping Zhao Hongqiang Wang Junting Liang Shengmin Xu Shaopeng Chen An Xu Lijun Wu 

机构地区：[1]Key Laboratory of High Magnetic Field and Ion Beam Physical Biology,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China. [2]Department of Biological and Environmental Engineering,Hefei University,Hefei 230601,China [3]Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province,Hefei 230031,China [4]Institute of Intelligent Machines,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China [5]School of Nuclear Science and Technology,University of Science and Technology of China,Hefei 230026,China

出　　处：《Journal of Environmental Sciences》2018年第8期133-143,共11页环境科学学报（英文版）

基　　金：supported by the CAS Strategic Priority Research Program(No.XDB14030502);the National Basic Research Program(973)of China(No.2014CB932002);the Natural Science Foundation of Anhui Province(No.1808085QB37);the Hi-Tech Research and Development Program(863)of China(No.2008AA062504);the National Natural Science Foundation of China(Nos.20977093,81273004,81301182,and 31470829);the Talent Research Foundation of Hefei University(No.16-17RC03);the Key Project of Natural Science Research of Anhui High Education Institutions(Nos.KJ2017A545,and KJ2017A546);the Project of Anhui Quality Engineering(No.2016msgzs060)

摘　　要：The Jialu River in China has been seriously polluted by the direct discharge of industrial and domestic wastewater. The predominant contaminants of the Jialu River and its adjacent groundwater were recently investigated. However, the potential genotoxic impact of polluted water on human health remains to be clarified. Here, we used human–hamster hybrid（AL） cells, which are sensitive for detecting environmental mutagens. We found that the cytotoxicity and mutagenicity of the groundwater in the Jialu River basin were influenced by the infiltration of the Jialu River. Hydrological periods significantly affected the cytotoxicity, but not the mutagenic potential, of surface and groundwater. Further, the mutagenic potential of groundwater samples located 〈 1 km from the Jialu River（S（M-2） water samples） was detected earlier than that of groundwater samples located approximately 20 km from the Jialu River（SN water samples）. Because of high cytotoxicity, the mutagenic potential of water samples from the Jialu River（S（M-1） water samples） was not significantly enhanced compared with that of untreated controls. To further assess the mutagenic dispersion potential, an artificial neural network model was adopted. The results showed that the highest mutagenic potential of groundwater was observed approximately 10 km from the Jialu River. Although further investigation of mutagenic spatial dispersion is required, our data are significant for advancing our understanding of the origin, dispersion,and biological effects of water samples from polluted areas.The Jialu River in China has been seriously polluted by the direct discharge of industrial and domestic wastewater. The predominant contaminants of the Jialu River and its adjacent groundwater were recently investigated. However, the potential genotoxic impact of polluted water on human health remains to be clarified. Here, we used human–hamster hybrid（AL） cells, which are sensitive for detecting environmental mutagens. We found that the cytotoxicity and mutagenicity of the groundwater in the Jialu River basin were influenced by the infiltration of the Jialu River. Hydrological periods significantly affected the cytotoxicity, but not the mutagenic potential, of surface and groundwater. Further, the mutagenic potential of groundwater samples located 〈 1 km from the Jialu River（S（M-2） water samples） was detected earlier than that of groundwater samples located approximately 20 km from the Jialu River（SN water samples）. Because of high cytotoxicity, the mutagenic potential of water samples from the Jialu River（S（M-1） water samples） was not significantly enhanced compared with that of untreated controls. To further assess the mutagenic dispersion potential, an artificial neural network model was adopted. The results showed that the highest mutagenic potential of groundwater was observed approximately 10 km from the Jialu River. Although further investigation of mutagenic spatial dispersion is required, our data are significant for advancing our understanding of the origin, dispersion,and biological effects of water samples from polluted areas.

关 键 词：Jialu River Water sample Flow periods Mutagenic potential Artificial neural network 

分 类 号：Q1[生物学—普通生物学]