右美托咪定通过腺苷A1受体调节大鼠压力反射敏感性  

Baroreflex sensitivity of rats can be regulated by dexmedetomidine via adenosine A1 receptor

作　　者：田磊 路凯 祝瑜 殷永强 钟毅 高鸿 TIAN Lei;LU Kai;ZHU Yu;YIN Yongqiang;ZHONG Yi;GAO Hong(Departmemt of Anesthesiology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学麻醉学院,贵阳市550004 [2]贵州医科大学附属医院麻醉科

出　　处：《临床麻醉学杂志》2018年第8期788-790,共3页Journal of Clinical Anesthesiology

基　　金：贵州省科技厅省校合作计划项目(黔科合LH字[2015]7438)

摘　　要：目的观察腺苷A1受体在右美托咪定调节压力反射敏感性(baroreflex sensitivity,BRS)中的作用。方法健康成年雄性SD大鼠32只,体重240~280g,按随机数字表随机分为四组:对照组(C组)、选择性腺苷A1受体阻断剂组(P组)、右美托咪定组(D组)、选择性腺苷A1受体阻断剂+右美托咪定组(PD组),每组8只。C组泵注生理盐水40 ml·kg^(-1)·h^(-1)负荷量15 min,维持泵注10 ml·kg^(-1)·h^(-1);P组腹腔注射选择性腺苷A1受体阻断剂8-环戊基-1,3-二丙基黄嘌呤(DPCPX)1mg/kg,泵注同C组方案的生理盐水;D组右美托咪定负荷量100μg/kg,维持量100μg·kg^(-1)·h^(-1)持续泵注;PD组腹腔注射DPCPX 1mg/kg并泵注右美托咪定,泵注剂量同D组。采用苯肾上腺素升压法于泵注前(T_0)、泵注后60min(T_1)和泵注后120min(T_2)测定BRS。结果与T_0时比较,T_1和T_2时D组和PD组BRS明显升高(P<0.05)。与C组和P组比较,T_1和T_2时D组和PD组BRS均明显升高(P<0.05)。与D组比较,T_1和T_2时PD组BRS明显降低(P<0.05)。结论右美托咪定可能通过腺苷A1受体增加大鼠BRS。Objective To investigate the role of adenosine A1 receptor in the regulation of baroreflex sensitivity（BRS）by dexmedetomidine in rats.Methods Thirty-two healthy male SpragueDawley rats,weighting 240-280 g,were randomly divided into 4 groups,with 8 rats in each group.The equal volume of normal saline was administered in group C as compared to expermental groups.Intraperitoneal injection of selective adenosine A1 receptor blocker 8-cyclopentyl-1, 3-dipropylxanthine（DPCPX）1 mg/kg was used first,and then the equal volume of normal saline was administered in group P.Dexmedetomidine 100μg/kg was administered within 15 minutes followed by infusion at 100μg·kg-1·h-1 in group D.Intraperitoneal injection of DPCPX 1 mg/kg was used first,and then dexmedetomidine 100μg/kg was administered within 15 minutes followed by infusion at 100μg·kg-1·h-1 in group PD.BRS was measured before（T0）,60 min after（T1）and 120 min after（T2）intravenous pumping of dexmedetomidine or normal saline.Results Compared with T0,the BRS was significantly higher in groups D and PD at T1 and T2（P 〈 0.05）.Compared with groups C and P,BRS was significantly higher in groups D and PD at T1 and T2（P 〈 0.05）.Compared with group D,the BRS was significantly lower in group PD at T1 and T2（P 〈 0.05）.Conclusion Dexmedetomidine can increase the BRS of rats with adenosine A1 receptor.

关 键 词：右美托咪定 腺苷A1受体 压力反射敏感性 

分 类 号：R614[医药卫生—麻醉学]