二次谐波成像技术在腱病动物实验研究中的应用综述  

Application of second harmonic generation imaging in tendinopathy research

作　　者：胡波[1,2] 薛曼 李春宝 高奉[1,5] 张贞 申学振[1] 张柏青[1] 刘雨丰 鹿鸣 刘玉杰 HU Bo;NUN Man;LI Chunbao;GAO Feng;ZHANG Zhen;SHEN Xuezhen;ZHANG Baiqing;LIU Yufeng;LU Ming;LIU Yujie(Department of Orthopedics,Chinese PLA General Hospital,Beijing 100853,China;The Second Department of Orthopedics,Beijing Chaoyang Integrative Medicine Emergency Medical Center,Beijing 100022,China;Beijing National Laboratory for Molecular Sciences,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 100049,China;National Institute of Sports Medicine,Beijing 100061,China)

机构地区：[1]解放军总医院骨科,北京100853 [2]北京朝阳中西医结合急诊抢救中心骨二科,北京100022 [3]中国科学院化学研究所分子科学国家研究中心,北京100190 [4]中国科学院大学,北京100049 [5]国家体育总局运动医学研究所,北京100061

出　　处：《解放军医学院学报》2018年第8期727-729,共3页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81601952)~~

摘　　要：二次谐波成像是利用非线性光学效应对组织进行成像的一种技术,具有无需染色、空间分辨率高、穿透力强、成像亮度和信噪比高、光漂白性小、无光毒性等优点,在生物医学研究领域已经得到广泛应用。肌腱组织主要成分为胶原纤维,其组织特性非常符合二次谐波成像介质的条件。本文简述了二次谐波成像的特点及其在当前腱病研究中的状况及遇到的困难,并简析了其在腱病研究中的应用前景。Second harmonic generation imaging（SHG-IM）, as a novel nonlinear optical imaging technique, has been widely used in biomedical research. This label-free imaging technique has many advantages, such as higher spatial resolution, greater depth of penetration, higher singal-noise ratio, less photobleaching, nonphototoxicity and so on. The tendon tissues are rich in collagen fibers, which exhibit endogenous SHG signals. Therefore, tendons can be well studied by SHG-IM technique. In this review, we have overviewed the characteristics of the SHG-IM technique, and discussed the difficulties and prospect of applications of SHG-IM in tendinopathy research.

关 键 词：二次谐波成像 肌腱病 胶原纤维 非线性光学效应 

分 类 号：R310[医药卫生—基础医学]