荧光原位杂交技术的发展及其在植物基因组研究中的应用  

Development of FISH and Its Application in Plant Genome Research

作　　者：刘玉玲[1] 刘林杰 彭仁海[1] Liu Yuling;Liu Linjie;Peng Renhai(Anyang Institute of Technology,Anyang,45500)

机构地区：[1]安阳工学院,安阳455000

出　　处：《分子植物育种》2018年第17期5696-5703,共8页Molecular Plant Breeding

基　　金：国家自然基金(31471548);国家重点研发计划(2016YFD0100203);河南省高等学校重点科研项目计划(18B210001)共同资助

摘　　要：荧光原位杂交(FISH)技术能够实现DNA序列在染色体上的物理定位,已经广泛应用于植物基因组研究中。本综述基于FISH技术分辨率和灵敏度的提高,从探针类型和靶标染色体类型两个方面总结了FISH技术的发展进程及不同FISH技术的应用优势和不足之处。在技术应用方面,总结了FISH技术在植物染色体识别、核型分析、系统进化及亲缘关系分析、异源染色质及转基因鉴定、基因定位和物理图谱构建等研究中的应用进展,并对其应用前景进行了展望,以期为植物基因组研究提供更多的研究思路。Fluorescence in situ hybridization（FISH） is a method to physically map DNA sequence on the chromosomes efficiently,which has been widely used in plant genomes researches.In this review,based on the improvement of the resolution and sensitivity of FISH,we summarized the progress of the FISH technique and the application advantages and deficiencies of different FISH technologies from two aspects of probe type and target chromosome type.In terms of technology application,we summarized the application progress of FISH technology in plant chromosome identification,karyotype analysis,system evolution and genetic relationship analysis,heterochromatin and transgenic identification,gene location and physical maps construction and other researches.We also prospected its application in the future so as to provide more study ideas for plant genome research.

关 键 词：荧光原位杂交(FISH)技术 染色体识别 核型分析 物理图谱 

分 类 号：Q943.2[生物学—植物学]