连香树胚性细胞悬浮系的建立和植株再生  

Establishment of Suspension Culture System of Embryogenic Cell and Plant Regeneration of Cercidiphyllum japonicum Sieb.

作　　者：邬洁丽 陈发菊 梁宏伟 Wu Jieli;Chen Faju;Liang Hongwei(Key Laboratory of Three Gorges Regional Plant Genetics ＆ Germplasm Enhancement（CTGU）,Biotechnology Research Center,China Three Gorges University,Yichang,44300)

机构地区：[1]三峡区域植物遗传与种质创新重点实验室(三峡大学),三峡大学生物技术研究中心,宜昌443002

出　　处：《分子植物育种》2018年第17期5738-5742,共5页Molecular Plant Breeding

基　　金：国家“十二五”科技支撑计划课题(2012BAD21B03)项目资助

摘　　要：本试验以连香树授粉120 d后未成熟种子子叶胚为外植体,开展胚性愈伤诱导增殖和悬浮培养体系的研究,初步建立了连香树胚性细胞悬浮系与植株再生体系。将连香树未成熟子叶胚接种在添加0.5 mg/L6-BA+1.0 mg/L NAA+1.0 g/L AC的1/2 MS基本培养基上,进行胚性愈伤组织诱导,获得的愈伤组织在0.1 mg/L 6-BA+0.1 mg/L NAA+0.5 g/L AC的1/2 MS固体培养基上进一步增殖培养。将得到的胚性愈伤组织置于添加5%聚乙二醇6000的1/2 MS的悬浮培养液中进行振荡培养,建立起细胞均一、增殖较快、稳定性较强的细胞悬浮系;将悬浮培养获得的胚性材料接种到添加5%香蕉汁的0.1 mg/L 6-BA+0.1 mg/L NAA+0.5 g/L AC的MS固体培养基中进行培养,可分化出的子叶期体胚,将获得的子叶胚转接到添加1.0 mg/L IBA的WPM固体培养基中,体胚萌发再生成植株。This research studied the induction and proliferation of embryogenic callus and the culture condition of regeneration system in Cercidiphyllum japonicum by using the cotyledon embryo of immature seeds 120 days after pollination as explant.We initially established embryogenic cells suspension system and plant regeneration system of Cercidiphyllum japonicum.The immature cotyledon embryo was inoculated with 1/2 MS solid medium added0.5 mg/L 6-benzylaminopurine（6-BA）,1.0 mg/L naphthalene acetic acid（NAA） and 1.0 g/L active carbon（AC）for induction and proliferation on 1/2 MS solid medium supplemented with 0.1 mg/L 6-BA,0.1 mg/L NAA and0.5 g/L AC.Then,the obtained embryogenic callus was incubated in a suspension culture medium of 1/2 MS supplemented with 5% polyethylene glycol 6000.Finally,we got a suspension system together with high stability,fast proliferation and uniform cells.The embryogenic material obtained by suspension culture was inoculated into MS solid medium supplemented with 5% banana juice and added with 0.1 mg/L 6-BA,0.1 mg/L NAA and 0.5 g/L AC,the cotyledon stage somatic embryo was differentiated.The obtained cotyledon embryos were transferred in WPM solid medium with 1.0 mg/L IBA.The somatic embryos germinated and regenerated into plants.

关 键 词：连香树 胚性细胞 悬浮培养 植株再生 

分 类 号：S792.99[农业科学—林木遗传育种]