硝酸盐胁迫下黄腐酸对小白菜活性氧代谢及相关基因表达的影响  

Effects of Fulvic Acid on Reactive Oxygen Metabolism and Related Gene Expression in Pakchoi under NO_3^- Stress

作　　者：庞强强[1] 孙光闻[2] 蔡兴来 周曼[1] 赵枢纽 李德明[1] Pang Qiangqiang;Sun Guangwen;Cai Xinglai;Zhou Man;Zhao Shuniu;Li Deming(Hainan Provincial Key Laboratory for Vegetable and Biology,Vegetable Research Institute for Hainan Provincial Academy of Agricultural Sciences,Haikou,571100;College of Horticulture,South China Agricultural University,Guangzhou,510642)

机构地区：[1]海南省农业科学院蔬菜研究所,海南省蔬菜生物学重点实验室,海口571100 [2]华南农业大学园艺学院,广州510642

出　　处：《分子植物育种》2018年第17期5812-5820,共9页Molecular Plant Breeding

基　　金：2017年海南省重大科技计划项目(ZDKJ2017001); 2016年海南省重点研发项目(ZDYF2016042)共同资助

摘　　要：以小白菜为试验材料,在水培条件下,研究叶面喷施不同浓度黄腐酸(0.08%,0.15%,0.25%)对硝酸盐胁迫下(120 mmol/L)小白菜活性氧代谢及相关基因表达的影响。结果表明,硝酸盐胁迫可引起小白菜叶片中MDA、O2-和H_2O_2含量的增加,生长受到抑制;硝酸盐胁迫下施用黄腐酸(fulvic acid,FA)能显著促进小白菜生长发育,提高膜稳定指数和抗氧化酶活性,增加脯氨酸和可溶性糖等渗透调节物质含量,减少O_2^-和 H_2O_2的积累,其中以处理Ⅳ(0.15%FA)处理效果最好;随黄腐酸浓度的增加,活性氧代谢相关基因Cu/Zn-SOD和POD的表达水平呈先升后降的趋势,CAT基因表达水平持续增加,APX基因表达水平则表现为先降后升随后下降的趋势,其中Cu/Zn-SOD、POD和APX基因表达水平均在处理Ⅳ(0.15%FA)时达到顶峰,CAT基因表达水平在处理Ⅴ(0.25%FA)时最高。适宜浓度的黄腐酸可有效缓解硝酸盐胁迫对小白菜所造成的伤害,增强其抗盐性。本研究通过喷施外源黄腐酸,研究其对硝酸盐胁迫下小白菜生长、活性氧、渗透调节物、抗氧化酶活性及相关基因表达的影响,为探讨黄腐酸调控蔬菜盐害提供理论依据。Using leaf spraying method,water culture experiment was carried out on the test material of pakchoi for investigating the effects of different concentrations of fulvic acid（0.08%,0.15%,0.25%） on reactive oxygen metabolism and related gene expression in pakchoi under NO3^- stress（120 mmol/L）.The results showed that NO3^- stress could increase the contents of MDA,O2-and H2O2 in pakchoi,and the growth were inhibited.Fulvic acid（FA） treatment under NO3^- stress could significantly promote the growth and development of pakchoi,improve the membrane stability index and antioxidant enzyme activity,increase the contents of percolating substances such as proline and soluble sugar,and reduce the accumulation of O2- and H2O2.Among them,0.15% FA treatment had the best effect.With the increase of the concentration of fulvic acid,the expression levels of the related genes Cu/Zn-SOD and POD of reactive oxygen metabolism showed the trend of first ascending and then descending.The level of CAT gene expression increased continuously,and the expression level of APX gene first decreased,then increased and finally decreased.The expression level of Cu/Zn-SOD,POD and APX genes all reached the peak when 0.15% FA（treatment Ⅳ） was processed,and the expression level of CAT gene was highest at 0.25% FA（treatment Ⅴ）.It suggested that fulvic acid in appropriate concentration could alleviate the damage of pakchoi under NO3^- stress through increasing the salt resistance.By spraying exogenous fulvic acid,the study investigated the its effects on the growth,reactive oxygen species,osmoregulation substance,antioxidant enzyme activities and the expression of related genes in pakchoi under NO3^- stress,which would provide a theoretical basis for the regulation of fulvic acid on vegetable salt damage.

关 键 词：黄腐酸 硝酸盐胁迫 小白菜 活性氧 基因表达 

分 类 号：S634.3[农业科学—蔬菜学]