二硫代氨基甲酸盐(酯)类(DTCs)杀菌剂在4种水果中的残留分析  

Residue analysis of dithiocarbamates(DTCs) fungicid in four kinds of fruits

作　　者：柯华[1] 郑申西[1] 林捷[1] KE Hua;ZHENG Shen-xi;LIN Jie(Fuzhou Center for Disease Control and Prevention,Fuzhou Fujian 350004,China)

机构地区：[1]福州市疾病预防控制中心,福建福州350004

出　　处：《中国卫生工程学》2018年第4期530-532,共3页Chinese Journal of Public Health Engineering

摘　　要：目的建立气相色谱法(FPD-S)测定4种水果中二硫代氨基甲酸盐(酯)类杀菌剂残留的检测方法。方法二硫代氨基甲酸盐(酯)类杀菌剂在Sn Cl2/HCl溶液的密闭顶空瓶中80℃水浴加热,消化反应生成二硫化碳,冷却到4℃用正己烷萃取,采用气相色谱法(FPD-S)检测器,宽口径毛细管柱,测定正己烷中二硫化碳含量,代表二硫代氨基甲酸盐(酯)类杀菌剂的残留量。结果采用该方法测定4种水果中福美锌、福美双、代森锰锌、代森联、丙森锌5种DTCs类杀菌剂残留,以二硫化碳含量计,标准曲线线性关系良好(r=0.997 7),二硫化碳添加水平在0.30、1.0μg/g 2种添加浓度下,回收率为76.7%~86.1%,相对标准偏差为5.8%~11.4%。方法的检出限为二硫化碳0.02 mg/kg,定量限为0.06 mg/kg。结论该方法对草莓、蓝莓、葡萄和猕猴桃4类水果基质中残留DTCs杀菌剂测定效果好,简单,快捷,重复性好,灵敏度高,无假阳性,测定结果能满足对该类杀菌剂残留限量(MRLs)的检测要求;也适合各种水果基质中DTCs杀菌剂残留的测定。Objective To determinate residual detection methods of dithiocarbamates（DTCs） fungicid in four kinds of fruits by Gas Chromatography（FPD-S）.Methods The fungicide was heated in a water bath at 80℃ in a sealed headspace bottle of SnCl_2/HCl solution,and then digestion reaction to produce carbon disulfide,cooled to 4℃ and extracted with n-hexane.The content of carbon disulfide in n-hexane was determinated by gas chromatography（FPD-S） used wide-caliber capillary column,representing the residual amount of dithiocarbamate fungicide.Results The method was used to determine the residue of five kinds of DTCs fungicides include Ziram,Thiram,Metiram,Propineb,Mancozeb in four kinds of fruits,calculated as the content of carbon disulfide.The standard curve had a good linear relationship（r=0.997 7）.At the addition of carbon disulfide levels at 0.30 μg/g,1.0 μg/g two kinds of addition concentrations,The recoveries were 76.7%-86.1%,the relative standard deviation was 5.8%-11.4%.The detection limit and quantization limit of this method as carbon disulfide were 0.02 mg/kg and 0.06 mg/kg respectively.Conclusion The method has good effect on the determination of residual DTCs in strawberry,blueberry,grape and kiwi fruit.The method is simple,quick and reproducible,and has high sensitivity and no false positive.The results of the assay can meet the detection requirements for the residual limit（MRLs） of the fungicide.It is also suitable for the determination of residues remaining in DTCs of various fruit substrates.

关 键 词：二硫代氨基甲酸盐(酯)类(DTCs) 杀菌剂 残留 水果 

分 类 号：O657.63[理学—分析化学] R16[理学—化学]