电针对慢性神经痛大鼠海马区高迁移率族蛋白1表达的影响  

Electroacupuncture Relieves Pain by Down-regulating Expression of Hippocampal High Mobility Group Protein 1 and Contents of TNF-α and IL-1 β in Rats with Chronic Neuropathic Pain

作　　者：王珂[1,2] 曾亮 周伊人 周映帆 赵冉 杨丽萍[3] 侯文光 WANG Ke;ZENG Liang;ZHOU Yi-ren;ZHOU Ying-fan;ZHAO Ran;YANG Li-ping;HOU Wen-guang(Institute of Clinical Immunology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine;Shanghai Key Laboratory of Health and Disease Genomics,Chinese National Human Genome Center at Shanghai;Department of Acupuncture-moacibustion,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳医院临床免疫研究所 [2]上海人类基因组研究中心上海市疾病与健康基因组学重点实验室 [3]上海中医药大学附属岳阳医院针灸科,上海200437

出　　处：《针刺研究》2018年第8期480-484,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81673756;81202767);上海市自然科学基金项目(No.16ZR 1424000);上海市进一步加快中医药事业发展三年行动计划项目(No.ZY 3-CCCX-2-1003;ZY 3-JSFC-1-1023);上海市卫生和计划生育委员会中医科研基金项目(No.2014LP 106A)

摘　　要：目的:观察电针对慢性神经痛大鼠海马区高迁移率族蛋白1(HMGB 1)及其下游效应促炎细胞因子的影响,探讨电针镇痛的作用机制。方法:雄性SD大鼠随机分为假手术组、模型组和电针组,每组12只。结扎大鼠左侧坐骨神经建立坐骨神经慢性结扎性损伤模型。电针组电针双侧"足三里""阳陵泉",每次30min,每日1次,共7d。检测各组大鼠左后足底机械痛阈(MWT),荧光定量PCR法和Western blot法分别检测海马HMGB 1基因和蛋白的表达水平,ELISA法检测海马区肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)的表达水平。结果:与假手术组比较,模型组MWT显著降低(P<0.001)。与模型组比较,造模第10天和第14天,电针组MWT显著升高(P<0.05,P<0.01)。与假手术组比较,模型组大鼠海马HMGB 1基因及蛋白表达水平显著增加(P<0.001),TNF-α和IL-1β蛋白表达水平也均显著升高(P<0.001)。电针7d后,与模型组比较,电针组海马区HMGB 1基因及蛋白表达水平显著降低(P<0.001,P<0.01),TNF-α和IL-1β蛋白表达水平显著降低(P<0.05)。结论:电针可明显提高慢性神经痛大鼠痛阈值,其作用机制可能与抑制大鼠海马区HMGB 1及其下游促炎细胞因子释放有关。Objective To observe the effect of electroacupuncture（EA）on expression of high mobility group protein 1（HMGB 1）and related downstream effectors of proinflammatory cytokines in the hippocampus in chronic neuropathic pain rats,so as to investigate its mechanism underlying neuropathic pain relief.Methods Male SD rats were randomized into sham,model,and EA groups,with 12 rats in each group.The neuropathic pain model was established by ligature of the left sciatic nerve to induce chronic constriction injury（CCI）.EA was applied to bilateral＂Zusanli＂（ST 36）and ＂Yanglingquan＂（GB 34）for 30 min,once daily for 7 days.The mechanical withdrawal threshold（WMT）was detected using an electronic von Frey anesthesiometer.The expression level of HMGB 1 in the hippocampus was determined using quantitative RT-PCR and Western blot,separately,and the contents of hippocampal TNF-αand IL-1β were detected by ELISA.Results Compared with the sham group,the MWT values were markedly decreased on day 7,10 and 14 after modeling in the model group（P〈0.001）.On day 10 and 14 after modeling,the MWT values were significantly up-regulated in the EA group relevant to those of the model group（P〈0.05,P〈0.01）.The expression levels of HMGB1 mRNA and protein,and the contents of hippocampal TNF-αand IL-1βwere markedly increased in the model group relevant to the sham group（P〈0.001）,and significantly down-regulated in the EA group relevant to the model group（P〈0.001,P〈0.01,P〈0.05）.Conclusion EA stimulation of ST 36-GB 34 can relieve pain in chronic neuropathic pain rats,which may be related to its actions in down-regulating the levels of HMGB 1 and its downstream proinflammatory cytokines TNF-αand IL-1βin the hippocampus.

关 键 词：电针镇痛 慢性神经痛 海马 高迁移率族蛋白1 肿瘤坏死因子-Α 白介素-1Β 

分 类 号：R245.97[医药卫生—针灸推拿学]