唐山地区医疗放射人员外周血淋巴细胞培养支原体污染的防控  

Prevention and control of mycoplasma contamination from peripheral blood lymphocyte culture of medical radiation workers in Tangshan

作　　者：赵俊鹏[1] 杨秋月 蔡超 赵阳 王玉君[1] 黄菲 张伟 ZHAO Jun-peng;YANG Qiu-yue;CAI Chao;ZHAO Yang;WANG Yu-jun;HUANG Fei;ZHANG Wei(Functional Branch,Tangshan Center for Disease Control and Prevention,Tangshan Hebei,063000,China;Microbiological Laboratory,Tangshan Worker＇s hospital,Tangshan Hebei,063000,China;School of Basic Medical Science,North China University of Science and Technology,Tangshan Hebei,063210,China)

机构地区：[1]唐山市疾病预防控制中心功能科,河北唐山063000 [2]唐山市工人医院微生物检验科,河北唐山063000 [3]华北理工大学基础医学院,河北唐山063210

出　　处：《职业与健康》2018年第16期2181-2184,共4页Occupation and Health

基　　金：河北省2018年度医学科学研究重点课题计划(20181383)

摘　　要：目的针对医疗放射人员外周血淋巴细胞畸变检测的淋巴细胞培养过程中耐药支原体污染防控,探索不同类别抗生素应用的可行性。方法根据美国国家临床试验室标准化委员会相关标准,采用不同种类抗生素对2017年10月—2018年2月在唐山市疾病预防控制中心功能科健康检查的医疗放射人员外周血进行淋巴细胞毒性试验及支原体污染清除试验,对样本检测结果进行统计学分析。结果细胞毒性试验显示,阿奇霉素组、多西环素组、莫西沙星组和克林霉素组的淋巴细胞畸变样本阳性率分别为5.61%、6.27%、7.59%和4.62%,链霉素对照组的样本阳性率为5.94%,各组样本阳性率比较差异无统计学意义(χ~2=2.073,P>0.05)。支原体污染清除试验显示,链霉素组、阿奇霉素组、多西环素组、莫西沙星组和克林霉素组支原体污染清除试验的样本阳性率分别为61.67%、21.67%、15.00%、3.33%和53.33%。其中莫西沙星组样本阳性率低于链霉素组、阿奇霉素组及克林霉素组,差异有统计学意义(χ~2=43.913,7.619,34.514,均P<0.05);阿奇霉素组样本阳性率低于链霉素组与克林霉素组,差异有统计学意义(χ~2=19.749,12.836,均P<0.05);多西环素组样本阳性率低于链霉素组与克林霉素组,差异有统计学意义(χ~2=27.638,19.599,均P<0.05)。其他各组样本阳性率比较差异无统计学意义(均P>0.05)。结论采用规定浓度莫西沙星、阿奇霉素和多西环素可较好防控医疗放射人员外周血淋巴细胞畸变试验中淋巴细胞培养的耐药支原体污染。[Objective]To explore the feasibility of preventing and controlling drug-resistant mycoplasma pollution in lymphocyte culture by different kinds of antibiotics for peripheral blood lymphocyte aberration detection of medical radiation workers.[Methods] According to the NCCLS standards,different kinds of antibiotics were used to carry out lymphocyte toxicity test and Mycoplasma pollution removal test on peripheral blood of medical radiators who had been occupational examined in functional branch of Tangshan Center for Disease Control and Prevention since October 2017-February 2018. The results were analyzed statistically. [ Results ] The sample positive rates of lymphocyte toxicity test were 5. 61 %, 6. 27 %, 7. 59 % and 4. 62 % in Azithromycin group,Doxycycline group,Moxifloxacin group and Clindamycin group,and 5.94% in Streptomycin control group.There was no significant difference between each group（χ^2=2.073,P〈0.05）. The sample positive rates of Mycoplasma pollution removal test were 61.67%,21.67%,15.00%,3.33% and 53.33% in Streptomycin group,Azithromycin group,Doxycycline group,Moxifloxacin group and Clindamycin group. The sample positive rate of Moxifloxacin group was lower than that of Streptomycin group,Azithromycin group and Clindamycin group,with significant differences（χ^2=43.913,7.619,34.514,all P〈0.05）. The sample positive rate of Azithromycin group was lower than that of Streptomycin group and Clindamycin group,with significant differences（χ^2=19.749,12.836,all P〈0.05）. The sample positive rate of Doxycycline group was lower than that of Streptomycin group and Clindamycin group,with significant differences（χ^2=27.638,19.599,all P〈0.05）. There was no significant difference in the sample positive rate between other groups（P〈0.05）. [Conclusion] Moxifloxacin,Azithromycin and Doxycycline of specified concentration can be used to prevent and control drug-resistant mycoplasma pollution in peripheral blood lymphocyte culture in peripheral blood lymphocyte aberration detection

关 键 词：医疗放射 支原体 链霉素 阿奇霉素 多西环素 莫西沙星 克林霉素 

分 类 号：R14[医药卫生—公共卫生与预防医学]