Light标准对海水浸泡体温过低症模型大鼠胸水性质的连续观察  

Continuous observation on the Light criteria of pleural effusion in hypothermic rats induced by seawater immersion

作　　者：奂剑波 陈丽娜 韩志海 宋立成 程浩 乔媛媛[2] 张达矜[2] 史成和[2] Huan Jianbo;Chen Lina;Han Zhihai;Song Licheng;Cheng Hao;Qiao Yuanyuan;Zhang Dajin;Shi Chenghe(Department of Respiratory and Critical Care Medicine,Navy General Hospital,Beijing 100048,China)

机构地区：[1]海军总医院呼吸与危重症医学科,北京100048 [2]海军总医院海战伤救治研究中心,北京100048 [3]解放军医学院研究生院 [4]安徽医科大学研究生院

出　　处：《中华航海医学与高气压医学杂志》2018年第3期129-134,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：军队后勤科研计划重点项目（BHJ14C009;BHJ16J011）

摘　　要：目的利用Light标准连续观察海水浸泡体温过低症SD大鼠胸水性质的变化，并分析其原因。方法100只雄性成年SD大鼠按数字表法随机均分为正常对照组（不做任何处理），低温实验组（20 ℃海水浸泡24 h），被动复温组1、2、3、4（20 ℃海水浸泡24 h＋被动复温，分别在复温后0、3、6、12 h处死），温水浴复温（warm water bath rewarming, wR）组1、2、3、4（20 ℃海水浸泡24 h＋wR，分别在复温后0、3、6、12 h处死），每组10只。检测血清、胸水总蛋白（total protein, TP），血清、胸水、肺组织匀浆乳酸脱氢酶（lactate dehydrogenase, LDH）浓度，计算Light标准数值。结果正常大鼠无明显胸水产生。低温性胸水LDH大于正常血清LDH，胸水/血清总蛋白比（total protein ratio, TPR）值小于0.5，乳酸脱氢酶比值（lactate dehydrogenase ratio, LDHR）小于0.6。复温后，低温性胸水的量逐渐减少；TPR与LDHR均逐渐升高，但胸水LDH与血清LDH、肺组织匀浆LDH变化趋势不同。wR对于低温性胸水的吸收更快，但差异无统计学意义。结论低温大鼠胸水Light标准中3项指标复温后均不断升高并达到渗出液标准，不一定反映胸膜的炎症损伤程度，可能的原因为复温后胸水的生成减少，而机体对水分的吸收大于对蛋白质的吸收。ObjectiveTo investigate the dynamic changes in the nature of the pleural effusion via Light criteria in hypothermic rats induced by seawater immersion and analyze possible mechanism involved.MethodsOne hundred male Sprague-Dawley rats were randomly divided into the normal control group （without any treatment） and hypothermia group exposed to 20 ℃ seawater for 24 hours. Then, the hypothermia group was sub-divided into the passive rewarming groups 1, 2, 3 and 4 and warm water bath active rewarming groups 1, 2, 3 and 4, each consisting of 10 animals. The passive rewarming groups had passive rewarming after exposure to 20 ℃ seawater for 24 hours, while the active rewarming groups had warm water bath rewarming following exposure to 20 ℃ seawater for 24 hours. Then, all the animals in the sub-groups were executed 0, 3, 6 and 12 hours after rewarming. Serum, pleural effusion, total protein （TP） in pleural effusion, concentrations of lung homogenate lactate dehydrogenase （LDH） were measured, and Light criteria were calculated.ResultsThere was no significant pleural effusion in the normal rats. LDH level in hypothermia-induced pleural effusion was higher than that in normal serum LDH, pleural effusion/total serum protein ratio （TPR） was lower than 0.5, and lactate dehydrogenase ratio （LDHR） was lower than 0.6. After rewarming, the amount of hypothermia-induced pleural effusion decreased gradually, while the levels of TPR and LDHR increased gradually. However, changes of LDH in pleural effusion were different with those in serum and lung homogenate. The warm water bath rewarming in the absorption of hypothermic pleural effusion was faster than passive rewarming, Warm water bath rewarming seemed to promote absorption of hypothermia-induced pleural effusion, but without statistical significance.ConclusionsThe 3 values of the pleural effusion criteria in hypothermic rats continuously increased following rewarming and reached the standards of effusion fluid, which did not necessarily reflect the serio

关 键 词：海水浸泡 体温过低症 大鼠 复温 胸水 Light标准 

分 类 号：R82[医药卫生—临床医学] R-332