水曲柳S6K基因的生物信息学及胁迫和激素下的表达分析  

Bioinformatics of S6K Genes in Fraxinus mandshurica and Their Expression Analysis under Stress and Hormone

作　　者：孙泓 赵兴堂[1,2] 刘璋 杨凯 王颖 詹亚光[1,2] SUN Hong;ZHAO Xing-Tang;LIU Zhang;YANG Kai;WANG Ying;ZHAN Ya-Guang(College of life science,Northeast Forestry University,Harbin 150040;State Key Laboratory of Forestry Genetics and Breeding of Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2018年第5期714-724,共11页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31270697);东北林业大学大学生创新创业训练计划项目(201710225035)~~

摘　　要：以所获取的水曲柳FmS6K基因(FmS6K1和FmS6K2)为对象,解析其核苷酸序列及其所编码蛋白质的基本信息,探索非生物胁迫和植物激素条件下基因的表达特征。结果表明,FmS6K1和FmS6K2基因长度分别为1 583和1 796 bp,分别编码480和483个氨基酸,二者均含有完整的开放阅读框。FmS6K1为两性蛋白,FmS6K2是亲水蛋白,二者均不含有信号肽。低温(4℃)和盐(NaCl)胁迫均影响FmS6K1和FmS6K2基因的表达,水曲柳根、茎、叶中的FmS6K1和FmS6K2基因对盐更加敏感,FmS6K2基因比FmS6K1基因对低温和盐胁迫的响应更为敏感;外源ABA、GA和IAA均能够影响FmS6K1和FmS6K2基因的表达,FmS6K1在水曲柳根、茎、叶中对外源的IAA响应更大;水曲柳根茎叶中FmS6K2基因均对外源ABA处理有显著上调应答,对外源GA的响应主要在根中,但对IAA的应答主要在茎和叶片中。上述结果表明FmS6K基因在调控植物生长发育和适应逆境胁迫方面扮演重要角色。The nucleotide sequences of FmS6K（FmS6K1 and FmS6K2 ） gene and the basic information of protein encoded by FmS6K gene were analyzed, and the characteristic of gene expression under abiotic stresses and plant hormones were studied. The length of FmS6K1 and FmS6K2 genes were 1 583 and 1 796 bp, encoding 480 and 483 amino acids, respectively, both of which contained a complete open reading frame. FmS6K1 is an unstable amphipathic protein, meanwhile FmS6K2 is a labile hydrophilic protein, neither of which has a signal peptide. The expression levels of FmS6K1 and FmS6K2 genes were affected by both low temperature（4℃） and salt（NaCl） stress. Compared to low temperature, FmS6K1 and FmS6K2 genes in roots, stems and leaves were more sensitive to salt stress. In FmS6K1 and FmS6K2 genes, the later was more sensitive to low temperature and salt stress. Exogenous ABA, GA and IAA could affect the expression of FmS6K1 and FmS6K2 genes. The expression level of FmS6K1 gene in roots, stems and leaves was higher in IAA treatment comparing to ABA and GA treatment. The response of FmS6K2 to exogenous GA and ABA was more obvious in root, while that to exogenous IAA and ABA was more significant in stem and leaf. The above results indicated that the FmS6K gene played an important role in regulating plant growth and development and adapting to adversity stresses.

关 键 词：S6K基因 生物信息学 信号诱导 水曲柳 基因表达 

分 类 号：S718.46[农业科学—林学] S792.41