白芨水提物对小鼠黑色素瘤B16F10细胞增殖与迁移的影响  

Effect of the aqueous extract of Bletilla striata on proliferation and migration of mouse melanoma B16F10 cells

作　　者：令狐浪[1] 李成龙 姚晓东[1] Linghu Lang;Li Chenglong;Yao Xiaodong(Teaching Demonstration Center of Phaarmaceutieal Experiment,School of Pharmaey,Zunyi Medical University,Zunyi Guizhou 563099,China)

机构地区：[1]遵义医学院药学院药学实验教学示范中心,贵州遵义563099

出　　处：《遵义医学院学报》2018年第4期408-412,417,共6页Journal of Zunyi Medical University

基　　金：贵州省中医药管理局中医药;民族医药科学技术研究项目(NO:QZYY-2016-063)

摘　　要：目的探讨白芨水提物对小鼠黑色素瘤B16F10细胞增殖与迁移的影响。方法使用水提醇沉法提取白芨粗多糖,同时保留有机小分子物质部分;采用CCK8试剂检测不同浓度的两部分提取物对B16F10细胞存活率及增殖的影响;细胞划痕检测提取物对B16F10细胞迁移的影响,以及ELISA法检测迁移相关蛋白的表达情况。结果与对照组相比,白芨水提物均能降低B16F10细胞的存活率和增殖能力,并与浓度呈正相关,其中多糖和有机小分子物质的半数抑制浓度(IC_(50))分别为51.14μg/mL和40.61μg/mL;此外,白芨水提物对细胞迁移也具有抑制作用,并能下调MMP-2和MMP-9的表达,与对照组相比,中、高浓度的下调作用具有显著性差异。结论白芨水提物能够抑制B16F10细胞的增殖与迁移能力,其作用可能通过下调迁移相关蛋白MMP-2和MMP-9的表达有关。Objective To explore the effect of aqueous extract of Bletilla striata on proliferation and migration of mouse melanoma B16F10 cells. Methods Crude polysaccharides of Bletilla striata were extracted by aqueous extraction and alcohol precipitation,while the part of organic small molecule was preserved. After treatment with different concentrations of extracts,the cell viability and proliferation were detected by CCK8 assay,the cell migration was determined by cell scratch,as well as the expressions of migration-related proteins were detected by ELISA assay. Results Compared with control group,B16F10 cell viability and proliferation were reduced in a dose-dependent manner,and the IC50 values of polysaccharide and organic small molecule were 51. 14 μg/mL and 40. 61 μg/mL,respectively. In addition,the cell migration activity was inhibited and the expressions of MMP-2 and MMP-9 proteins were down-regulated after cultured with aqueous extract of Bletilla striata. Compared with control group,the down-regulation effects of medium and high concentration groups were significantly different. Conclusion The aqueous extract of Bletilla striata can inhibit the proliferation and migration of B16F10 cells,which may be related to the down-regulation of the protein levels of MMP-2 and MMP-9.

关 键 词：白芨 水提物 B16F10细胞 细胞增殖 细胞迁移 

分 类 号：R963[医药卫生—微生物与生化药学]