2015～2016年开封市儿童感染肠道病毒血清型监测结果分析  

Analysis of serological surveillance of enteroviruses in children infected with Kaifeng from 2015 to 2016

作　　者：王娜[1] 程倩倩[1] 宋晓敏 王芳 WANG Na;CHENG Qian-qian;SONG Xiao-min(Kaifeng Center for Disease Control and Preventio;Childrens Hospital of Kaifeng City,Henan Kaifeng 475000)

机构地区：[1]开封市疾病预防控制中心,河南开封475000 [2]开封市儿童医院,河南开封475000

出　　处：《医学检验与临床》2017年第12期10-12,6,共4页Medical Laboratory Science and Clinics

摘　　要：目的：分析2015～2016年开封市儿童感染肠道病毒血清型监测结果。方法：采集2015～2016年在开封市儿童医院治疗的689例儿童肠道病毒感染患儿的眼结膜分泌液、粪便、血清中的肠道病毒作为标本，使用全自动核酸提取仪将核酸提取出来后采用荧光定量RT-PCR（逆转录一聚合酶链式反应）识别病毒标本体内的mRNA，并扩增VP1区mRNA片段。结果：对提取出的病毒进行构成比例分析发现，病毒共有10种，其中所占比例较多的四种病毒分别为：柯萨奇病毒A型[CA16（33．9％）]、肠病毒[EV71（33．2％）]、柯萨奇病毒A型[CA10（12．2％）]、柯萨奇病毒A型[CA6（6．9％）]；观察患者的年龄、性别比例可发现，男性患者要比女性患者更敏感，更易感染肠道病毒血清型，患者主要感染年龄为1～4岁，因此，应对在此年龄阶段的儿童加强防护；对患者感染肠道病毒的严重程度进行分析发现，患者多数为在门诊就可治疗，仅有少数患者需住院治疗，以感染CA16的患者为例，其中86．8％的患者仅需门诊治疗，8．1％的患者需要住院治疗，5．1％的患者需在重症监护室中治疗，其差异具有统计学意义（P〈0．05）。结论：2015～2016年两年间开封市流行的肠道病毒血清型的种类较少，大部分患者感染疾病较轻，仅需普通门诊即可治疗。Objective： This study aims to observe the results of serological surveillance of enterovirus infection in children in Kaifeng City from 2015 to 2016. Methods： A total of 689 children with enterovirus infection were enrolled in Childrens Hospital of Kaifeng City from 2015 to 2016, and the samples were collected from the conjunctival secretions, feces and blood samples of the intestinal virus. The nucleic acid was extracted by using the automatic nucleic acid extractor and the fluorescence Quantitative RT-PCR （reverse transcription -polymerase chain reaction） to identify the virus in vivo mRNA, and amplification of VP1 region mRNA fragments. Results： The results showed that there were 10 viruses, among which the four viruses were CA16 （33.9%） , EV71 （33.2%） , CA10 （12.2%） , CA6 （6.9%） ; observation of the patient＇s age and sex ratio of the virus can be found in male patients than female patients are more sensitive, more susceptible to enterovirus serotype, the main infection age of 1-4 years, therefore, Stage of the children to strengthen the protection; the severity of the infection of patients with enterovirus analysis found that most patients can be treated in the outpatient, only a small number of patients need hospitalization, to CA16 patients, for example, of which 86.8% of patients only Outpatient treatment, 8.1% of patients need hospitalization, 5.1% of patients in the intensive care unit, the difference was statistically significant （P〈0.05） . Conclusion： There were fewer types ofenterovirus serotypes in Kaifeng City during the two years from 2015 to 2016, and most of the patients had mild infection and needed only general outpatient treatment.

关 键 词：儿童感染 肠道病毒血清型 监测结果 

分 类 号：R512.5[医药卫生—内科学]