ICP-MS及AAS法测定川明参的无机营养元素  

Assay of Inorganic Nutrient Elements in Chuanmingshen violaceum Roots by ICP-MS and AAS

作　　者：杨晓阳 岳媛 黄红[1] 梁士贤[3] 林芷珊 李晶[3] 吕光华[1] YANG Xiao-yang;YUE Yuan;HUANG Hong;LEUNG Kelvin SY;LUM Tsz Shan;LI Jing;LU Guang-hua(Key Laboratory of Standardization of Chinese Herbal Medicine in Ministry of Education,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Chongqing Academy of Chinese Medicine,Chongqing 400065,China;Department of Chemistry,Hong Kong Baptist University,Hong Kong)

机构地区：[1]成都中医药大学药学院/中药材标准化教育部重点实验室,四川成都611137 [2]重庆市中药研究院,重庆400065 [3]香港浸会大学化学系

出　　处：《中药材》2017年第12期2788-2794,共7页Journal of Chinese Medicinal Materials

基　　金：四川省科技支撑计划项目(2015SZ0040)

摘　　要：目的:建立川明参药材中无机营养元素含量的测定方法,并比较不同产地川明参的营养元素。方法:从四川省8个川明参种植县/区采集23份川明参样品,根据地理位置,将其产地分为A(德阳市中江县和成都市金堂县和青白江区)、B(绵阳市盐亭县)、C(南充市阆中市、巴中市巴州区、广元市苍溪县)、D(达州万源市)产区。采用微波消解-ICP-MS及AAS法测定这些样品中13种营养元素的含量,并运用主成分分析法及t检验法对其所含元素进行评价。结果:23份川明参样品中13种无机营养元素的含量均在一定范围之内,平均值分别为0.026μg/g(Li)、6.105μg/g(B)、7.638μg/g(Mn)、56.139μg/g(Fe)、0.031μg/g(Co)、3.695μg/g(Zn)、0.041μg/g(Se)、7.252μg/g(Sr)、0.022μg/g(Mo)、115.417μg/g(Na)、785.896μg/g(Mg)、7 356.074μg/g(K)、282.205μg/g(Ca)。尽管这些不同产地样品中元素的含量不同,但各自的指纹图谱却呈相似的波浪式分布态势。t检验结果表明,Se、Zn、Mn、Co、Fe、Mg元素的含量在4个产区之间有显著性差异,其余元素含量差异不显著。主成分分析法提取前6个因子的综合得分模型为F=3.295C_1+2.341C_2+2.131C_3+1.362C_4+1.061C_5+0.734C_6。4个产区综合得分由高至低的顺序为D(0.917,n=5)、A(0.180,n=6)、B(-0.381,n=3)、C(-0.400,n=9)。结论:ICP-MS法和AAS法能够快速、准确的测定川明参中营养元素的含量。以无机营养元素为指标,川明参的营养价值较高,为滋补佳品。从无机营养元素的含量考虑,D产区(达州万源市)为栽培川明参的适宜产区。Objective： To develop a method for determination of inorganic elements in Chuanmingshen violaceum,and to evaluate the nutrient value of herbal samples among different cultivation areas. Methods： A total of 23 Chuanmingshen violaceum samples were collected from eight counties in Sichuan. The cultivation area of these samples were assigned as four producing areas,i. e. A（ Zhongjiang County,Jintang County and Qingbaijiang District）,B（ Yanting County）,C（ Langzhong City,Bazhou District and Cangxi County） and D（ Wanyuan City） based on the geographical sites and ecological condition. The 13 inorganic nutrient elements were quantified in these Chuanmingshen violaceum samples by inductively coupled plasma-mass spectrometry（ ICP-MS） and atomic absorption spectrophotometry（ AAS）. The amounts of inorganic elements and nutrient value were compared and evaluated among these herbal samples and producing areas by principal component analysis（ PCA） and t-test. Results： The contents of the 13 inorganic elements in the 23 herbal samples were within a certain range. The mean were 0. 026 μg/g（ Li）,6. 105 μg/g（ B）,7. 638 μg/g（ Mn）,56. 139 μg/g（ Fe）,0. 031 μg/g（ Co）,3. 695 μg/g（ Zn）,0. 041 μg/g（ Se）,7. 252 μg/g（ Sr）,0. 022 μg/g（ Mo）,115. 417 μg/g（ Na）,785. 896 μg/g（ Mg）,7356. 074 μg/g（ K） and 282. 205 μg/g（ Ca）. Although the contents of these elements in different Chuanmingshen violaceum samples were not consistent,their fingerprints were similar in a regular wave profile,which was a characteristic for its genuine identification. The results of t-test analysis indicated that Se,Zn,Mn,Co,Fe,Mg contents were significantly different（ P 0. 05） among the four producing areas. The differences of amounts for other elements were not significant（ P0. 05）. Six factors were extracted and used to comprehensively evaluate the nutrient value for a specific Chuanmingshen violaceum sample by PCA analysis. The comprehensive function was F =3. 295

关 键 词：川明参 无机元素 ICP-MS AAS 主成分分析 

分 类 号：R284.1[医药卫生—中药学]