小鼠骨髓细胞及骨髓基质细胞混合输注对骨髓移植后造血重建的影响  

The Effect of Mixed Infusion of Bone Marrow Cells and Bone Marrow Stromal Cells on Hematopoietic Reconstitution in Lethally Irradiated Mice

作　　者：刘静[1] 纪树荃[1] 秦凤华[2] 陈惠仁[1] 柳小兰 耿玉 

机构地区：[1]空军总医院血液科,100036 [2]北京放射医学研究所

出　　处：《空军总医院学报》1999年第4期196-198,共3页Journal of General Hospital of Air Force,PLA

摘　　要：观察小鼠骨髓细胞及骨髓基质细胞混合输注对骨髓移植后造血重建的影响。给予受致死剂量照射的Ｂａｌｂ／ｃ小鼠输注同基因骨髓细胞１×１０７ 个及经体外扩增的同基因原代骨髓基质细胞 ２×１０５ 个，与单纯骨髓移植组比较，移植后第１４ 天外周血白细胞、血小板回升较快，第１５ 天、第２０ 天ＢＦＵ－ＧＭ、ＢＦＵ－Ｅ、ＣＦＵ－Ｅ、ＣＦＵ－Ｓ明显高于后者，第２０ 天已达正常。　结论：原代骨髓基质细胞不仅是可以移植的，而且能够加快造血重建，提高移植效果。To observe the effect of mixed infusion of bone marrow and bone marrow stromal cells on hematopoietic reconstitution in lethally irradiated mice. Balb/c mice lethally irradiated received 1×10 7 syngeneic bone marrow cells and 2×10 5 syngeneic bone marrow stromal cells via the intravenous rout. As compared with simple BMT group, the WBC and the BPC in peripheral blood recover very quickly on day 14 after BMT. The numbers of CFU GM, BFU E,CFU E,CFU S are higher than that of the simple BMT group on day 15 and day 20 after BMT. Conclusion: Primary expanded bone marrow stromal cells not only is transplantable, but also can accelerate hematopoietic reconstitution.

关 键 词：骨髓基质细胞 造血重建 骨髓移植 骨髓细胞 

分 类 号：R457.7[医药卫生—治疗学] R329.2[医药卫生—临床医学]