CIH对舌下神经核中H_2S合成酶影响的初步研究  

The changes on density of hydrogen sulfide synthase in hypoglossal motor nucleus in rats of chronic intermittent hypoxia

作　　者：李佳宸[1] 史雅文[1] 朱华 张希龙[3] 殷敏[1] 程雷[1] LI Jiacheng;SHI Yawen;ZHU Hua;ZHANG Xilong;YIN Min;CHENG Lei(Department of Otorhinolaryngology,The First Affiliated Hospital,Nanjing Medical University,Nanjing,Jiangsu,210029,China)

机构地区：[1]南京医科大学第一附属医院耳鼻咽喉科,南京210029 [2]南京农业大学医院耳鼻咽喉科 [3]南京医科大学第一附属医院呼吸科

出　　处：《中国中西医结合耳鼻咽喉科杂志》2018年第4期245-249,263,共6页Chinese Journal of Otorhinolaryngology in Integrative Medicine

基　　金：江苏省卫计委六个一工程(LGY2017070)

摘　　要：目的探讨慢性间歇性缺氧(chronic intermittent hypoxia,CIH)状态下,大鼠舌下神经核中3种硫化氢合成酶(CBS,CSE,3MST)表达量的变化。方法将12只雄性Sprague Dawley(SD)大鼠(8周龄,体重180~200 g)随机均分为CIH组和常氧对照组。CIH组大鼠放置于氧浓度5%-21%之间循环的低氧箱中,对照组大鼠培养箱的氧浓度保持在21%。5周后观察大鼠舌下神经核中CBS,CSE,3MST三种酶的表达量。结果 Western blot分析显示CIH组CBS和3MST在舌下神经核中的蛋白表达量均低于对照组(P<0.05)。RT-PCR分析显示CIH组CBS和3MST在舌下神经核中的m RNA表达量低于对照组(P<0.05),CSE m RNA在两组中的表达量没有统计学差异(P>0.05)。结论 CBS与3MST-H2S通路可能参与调节舌下神经相关的呼吸活动和保护舌下神经核免受CIH诱导损伤,提示CBS与3MST-H2S通路可能是OSA的重要发病机制之一。Objective To study the changes of 3 kinds of hydrogen sulfide synthases（CBS, CSE and 3 MST） in hypoglossal nuclei induced by chronic intermittent hypoxia（CIH） in rat. Methods Adult male Sprague-Dawley（SD） rats（n=12） were randomly and meanly divided into two groups（the CIH and the control group）. The rats of the CIH group were fed in low oxygen cabins（the fraction of oxygen volume circulating between 5% and 21%）. The control group was fed in atmospheric environment cabins. After 5 weeks, the expression of 3 kinds of synthase in hypoglossal nuclei were detected by Western blot and RT-PCR. Results The protein expression of CBS and 3 MST in the CIH group was significantly lower than that in the controls（P〈0.05）. Meanwhile, the m RNA expression of CBS and 3 MST in the CIH group was also lower than that in the control group（P〈0.05）. However, there was no statistical difference in the expression of CSE m RNA between the two groups（P〈0.05）. Conclusion CBS and 3 MST-H2 S pathways may be involved in regulating hypoglossal nerve-related respiratory activity and protecting hypoglossal nucleus from CIH-induced injury, suggesting that CBS and3 MST-H2 S pathway may be one of the important pathogenesis for OSA.

关 键 词：慢性间歇性缺氧 舌下神经核 硫化氢合成酶 

分 类 号：R766[医药卫生—耳鼻咽喉科]