烟草半胱氨酸蛋白酶的基因和蛋白序列分析  

Gene and Protein Sequence Analysis of Cysteine Protease in Tobacco

作　　者：殷雪艳 杨柳[1] 罗万麟 赵爽 刘彪 王光云 YIN Xueyan;YANG Liu;LUO Wanlin;ZHAO Shuang;LIU Biao;WANG Guangyun(Liangshan Tobacco Company,Xichang,Sichuan 615000,China)

机构地区：[1]凉山州烟草公司,四川西昌615000

出　　处：《天津农业科学》2018年第9期1-4,共4页Tianjin Agricultural Sciences

摘　　要：以ABW71226.1、BAA96501.1、ACB70409.1、AAW78660.1和ADV41672.1为材料,采用生物数据分析工具对烟草的半胱氨酸蛋白酶的基因和蛋白序列进行分析,分别以GSDS、MEME和Signal P 4.1工具对其基因结构、保守基序和信号肽进行检测。结果显示,Nt CP-1和Nt CP-2基因具有7个外显子,Nt CP-3和Nt CP-4基因含有4个外显子,Nt CP-5基因具有3个外显子,Nt CP-1、Nt CP-2和Nt CP-5基因具有上游序列,Nt CP-1、Nt CP-2、Nt CP-4和Nt CP-5基因具有下游序列;检测Nt CP共有5个Motif,其核心保守基序区为Motif-1—Motif-2—Motif-4,且位于木瓜蛋白酶家族半胱氨酸蛋白酶结构域中,Motif-1和Motif-2含有半胱氨酸和谷氨酰胺;Nt CP均属于信号肽,Nt CP-1和Nt CP-2的信号区分布相同(1~22),Nt CP-3和Nt CP-4的信号区分布相同(1~20),Nt CP-5的信号区为1~29。ABW71226.1, BAA96501.1, ACB70409.1, AAW78660.1 and ADV41672.1 were as experimental materials, the tobacco cysteine protease genes and protein sequences were analyzed using biological data analysis tools including GSDS, MEME and Signal P4.1. The results showed that Nt CP-1 and Nt CP-2 genes had 7 exons, Nt CP-3 and Nt CP-4 genes contained 4 exons, Nt CP-5 gene had 3 exons, Nt CP-1, Nt CP-2 and Nt CP-5 genes had the upstream sequence, Nt CP-1, Nt CP-2, Nt CP-4 and Nt CP-5 genes with the downstream sequence. 5 motifs were detected in Nt CP, in which the core conserved motif region was Motif-1-Motif-2-Motif-4, and located in cysteine protease domain of the papain family, Motif-1 and Motif-2 contained cysteamine acid and glutamine. Nt CP belonged to the signal peptide, the signal area of Nt CP-1 and Nt CP-2 were the same（1~22）; the signal regions of Nt CP-3 and Nt CP-4 were identical（1-20）, and the signal region of Nt CP-5 was 1 ~ 29.

关 键 词：烟草 半胱氨酸蛋白酶 基因序列 蛋白序列 

分 类 号：S572[农业科学—烟草工业]