RP-HPLC-PDAD法测定青叶胆中齐墩果酸和熊果酸的含量  

CONTENT DETERMINATION OF OLEANOLIC ACID IN SWERTIA MILEENSIS BY RP-HPLC-PDAD

作　　者：王博龙 张军雷[1,2] 邹盛勤 WANG Bo-long;ZHANG Jun-lei;ZOU Sheng-qin(Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines,Yichun,Jiangxi 33600,China;College of Chemistry and Bioengineering,Yichun University,Yichun,Jiangxi 336000,China)

机构地区：[1]江西省天然药物活性成分研究重点实验室,江西宜春336000 [2]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《井冈山大学学报（自然科学版）》2018年第3期84-87,共4页Journal of Jinggangshan University (Natural Science)

基　　金：国家"863"计划重点项目(2002AA2Z3217);江西省研究生创新专项资金项目(YC2013-S286)

摘　　要：目的建立了青叶胆中齐墩果酸和熊果酸的反相高效液相色谱-光电二极管阵列检测器(RP-HPLC-PDAD)定量分析方法。方法采用95%乙醇为溶剂超声提取,色谱柱为Kromasil C18柱(4.6mm×250 mm,5μm),柱温30℃。以甲醇∶水∶磷酸(88∶12∶0.15,v/v/v)为流动相等度洗脱,流速0.9 m L·min-1,采用光电二极管阵列检测器进行检测,检测波长210 nm。结果齐墩果酸进样量在0.1048~2.6200μg时,与峰面积呈良好的线性关系(r=0.9999),平均回收率为96.9%,RSD为1.7%(n=9);熊果酸进样量在0.2304~5.7600μg时,与峰面积呈良好的线性关系(r=0.9999),平均回收率为97.5%,RSD为1.5%(n=9)。结论方法准确,操作简便,数据可靠,可用于青叶胆中齐墩果酸和熊果酸的含量测定。Objective： A precise and sensitive method for the determination of oleanolic acid and ursolic acid in Swertia mileensis was developed by RP-HPLC-PDAD. Methods： The two compounds were extracted by ultrasonic wave aided method with 95 % ethanol solution, and separated on a Kromasil C18 column（5 μm 4.6 mm × 250 mm） at 30 ℃. A isocratic program was carried out at a flow rate of 0.9 m L·min-1 using methanol-water-phosphoric acid（88：12：0.15, v/v/v） as the mobile phase. Results： The photodiode array detector was used for qualitative and quantitative analysis and the detection wavelength was set at 210 nm. Oleanolic acid and ursolic acid showed good linear relationship with the peak area（r = 0.99999） at the range of 0.1048~2.6200 μg and 0.2304~5.7600 μg, respectively with the respective recovery rates of 96.9% and 97.5%, and RSD of 1.7 %（n = 9） and 1.5 %（n = 9）. Conclusion： The proposed method is accurate, simple and promises to be applicable for the determination of oleanolic acid and ursolic acid in S. mileensis.

关 键 词：青叶胆 齐墩果酸 熊果酸 高效液相色谱法 光电二极管阵列检测器 

分 类 号：R284.2[医药卫生—中药学]