含药肠吸收液:一种新的中药体外药理实验方法  

Drug-containing intestinal absorption solution: A new in vitro pharmacological method of Chinese materia medica

作　　者：张旻昱 龚慕辛[1] 杨洪军[2] ZHANG Min-yu;GONG Mu-xin;YANG Hong-jun(Beijing Key Laboratory of TCM Collateral Disease Theory Research,School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]首都医科大学中医药学院中医络病研究北京市重点实验室,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中草药》2018年第15期3457-3462,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划课题(2011BAI07B08);国家重大新药创制重大科技专项课题(2014ZX09201021-009);国家自然科学基金青年基金项目(81703840)

摘　　要：中药化学成分复杂,在体外药理实验中如何进行中药质量控制,揭示中药的作用机制,辨析其药效物质基础是值得关注的问题。近年来,含药肠吸收液作为一种新的中药体外药理活性评价方法开始运用于科研实验。阐述了含药肠吸收液运用于中药体外实验的方法流程,总结了目前其在中药体外药理实验中的应用情况,比较分析其应用优势。含药肠吸收液对于中药及中药复方质量评价、作用机制的解析以及药效物质基础的辨识具有重要的促进作用,同时具有操作方法简便、应用成本较低等优势,为含药肠吸收液在中药体外实验的应用和推广提供了依据,也为中药药理体外研究提供参考方法。The chemical composition of Chinese materia medica（CMM） is complex. It is crucial to carry out quality control in pharmacological experiment in vitro of CMM, understand its mechanism, and identify its pharmacodynamic material basis. In recent years, drug-containing intestinal absorption solution（DCIAS）, as a new method for evaluating in vitro pharmacological activity, has been used in research of CMM. This article detailed the methodology and development of applying DCIAS to in vitro experiments of CMM with a comparison to other methods. The results showed that DCIAS played an important role in quality evaluation, mechanism analysis and material basis identification of CMM and Chinese herbal compound prescriptions. Meanwhile, DCIAS has the advantages of being simple to operate and cheap to apply. This study provides a basis for the application of DCIAS and a reference for in vitro pharmacological study of CMM.

关 键 词：含药肠吸收液 中药 体外实验 质量控制 作用机制 有效成分辨析 

分 类 号：R285[医药卫生—中药学]