CRISPR-Cas系统的抗耐药菌应用  

The application of CISPR-Cas system in antibacterial resistance

作　　者：杨萍[1,2] 孙兵兵 杨俊杰 杨晟[4] 蒋宇[4] Yang Ping;Sun Bing-bing;Yang Jun-jie;Yang Sheng;Jiang Yu(School of Pharmacy,Fudan University,Shanghai 201203;Shanghai Institute of Pharmaceutical Industry,Shanghai 201203;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240;Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032)

机构地区：[1]复旦大学药学院,上海201203 [2]上海医药工业研究院,上海201203 [3]上海交通大学药学院,上海200240 [4]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《中国抗生素杂志》2018年第8期927-931,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金面上项目(No.81573329)

摘　　要：病原菌对抗生素的耐受对临床治疗造成严重威胁。CRISPR-Cas系统具有高度的靶标基因序列特异性与方便的可编程性,已被尝试用来控制耐药病原细菌。用噬菌体传递CRISPR-Cas系统切割耐药细菌特有靶标,可恢复其对抗生素的敏感或直接致其死亡。更吸引人的应用则是用CRISPR-Cas系统编辑噬菌体基因组,使其具备期望的抗菌性能。The tolerance of pathogens to antibiotics poses a serious threat to clinical treatment. The CRISPR-Cas system has a high specificity of target gene sequence and convenient programmability and has been tried to control drug-resistant pathogenic bacteria. The use of the phage-delivering CRISPR-Cas system to cleave specific targets of resistant bacteria restores sensitivity to antibiotics or directly causes death. A more attractive application is to use the CRISPR-Cas system to edit the phage genome for desired antibaial propertiecters.

关 键 词：CRISPR-Cas 噬菌体 抗菌治疗 

分 类 号：R978.1[医药卫生—药品]