Streptomyces hygroscopicus K2509利用离子交换废水制备培养基发酵生产井冈霉素A  

Validamycin A production by Streptomyces hygroscopicus K2509 using ion-exchange waste water

作　　者：邹维 夏建荣 刘华梅 刘守德 万俊 潘渝 周莉 郭美锦[1,3] 庄英萍 Zou Wei;Xia Jian-rong;Liu Hua-mei;Liu Shou-de;Wan Jun;Pan Yu;Zhou Li;Guo Mei-jin;Zhuang Ying-ping(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237;Wuhan Kernel Bio-tech Co.,Ltd Hubei,Wuhan 430074;Shanghai Biological Manufacturing Technology Innovation Center,Shanghai 200237)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]武汉科诺生物科技股份有限公司,武汉430074 [3]上海生物制造技术协同创新中心,上海200237

出　　处：《中国抗生素杂志》2018年第8期1043-1048,共6页Chinese Journal of Antibiotics

基　　金：国家高技术研究与发展计划(863计划)(No.2012AA021201)

摘　　要：本文筛选出一株对离子交换废水耐受性能较好的井冈霉素A发酵菌株Streptomyces hygroscopicus K2509。通过测定总糖、还原糖、发酵液电容、井冈霉素A产量等发酵参数,用摇瓶试验对比了S.hygroscopicus K2509和出发菌株S.hygroscopicus K18的差异,用15L罐试验验证了两菌株在离子交换废水配制培养基中的代谢特性。在摇瓶实验中,S.hygroscopicus K2509井冈霉素A产量低于出发菌株,但其表现出较好的离子交换废水耐受能力;在15L发酵罐实验中,S.hygroscopicus K18和K2509井冈霉素A产量分别为18.9和20.2g/L,产率分别为0.45和0.31g/L·h。新筛选菌株可在离子交换废水配制培养基中实现井冈霉素A正常发酵,可有效利用井冈霉素A提取工序所产生的废水,降低产品成本。Objective To screen out a new validamycin A fermentation strain Streptomyces hygroscopicus K2509 with high tolerance to ion exchange waste water. Methods We compared fermentation parameters, including total sugar, reducing sugar, broth capacitanceand validamycin A yield difference between strain K2509 and the original strain K18. Results In shaking flasks, the yield ofvalidamycin A for strain I（2509 was lower than that produced by strain K18, but K2509 had better tolerance when it was cultivated with ion-exchange waste water. In a 15L bioreactor, the yields of validamycin A were 18.9g/L with a productivity of 0.45g/L/h for S. hygroscopicus K18 and 20.2g/L with a productivity of 0.31g/L/h for S. hygroscopicus K2509, respectively. Conclusion The new strain could effectively produce the validamycin A using ion-exchange waste water, leading to a reduction in the sewage discharge and manufacture cost.

关 键 词：井冈霉素A 离子交换废水 发酵过程分析 污水减排 

分 类 号：R978.1[医药卫生—药品]