基于HPLC-Q/TOFMS/MS技术的菟丝子醇提物的大鼠体内成分分析  

Qualitative analysis of metabolites of ethanol extract of Cuscuta chinensis by HPLC-Q/TOF MS/MS

作　　者：王靖雅 孙向明 宋辉[1] 胡杨 徐蓓蕾[1] 阎新佳 王昕蕾 张萌萌 李文兰[1] WANG Jing-ya;SUN Xiang-ming;SONG Hui;HU Yang;XU Bei-lei;YAN Xin-jia;WANG Xin-lei;ZHANG Meng-meng;LI Wen-lan(School of Pharmacy,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《中草药》2018年第16期3770-3775,共6页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省自然科学基金重点项目(ZD2017014)

摘　　要：目的对ig给予具有拟雌激素活性的菟丝子醇提物后大鼠的体内化学成分进行定性分析。方法应用HPLC-Q/TOF MS/MS技术,对大鼠尿液中的原型成分及代谢产物进行解析。结果在含药尿液中表征出14个化学成分,鉴定出12个化学成分,其中包括6个原型成分,分别为山柰酚-3-β-D-葡萄糖醛酸苷、6-O-(反式)对香豆酰基-β-D-呋喃果糖-(2→1)-α-D-吡喃葡萄糖苷、山柰酚-7-鼠李糖苷、绿原酸、芹菜素、槲皮苷;6个代谢成分,分别为对羟基桂皮酸、对羟基苯丙酸、异鼠李素、山柰酚-3-O-葡萄糖苷、乙酰化咖啡酸、槲皮素硫酸酯。结论应用HPLC-Q/TOF MS/MS技术能够简单、快速地分析大鼠ig菟丝子醇提物后尿液中的原型成分及代谢产物,为进一步阐明其药效物质基础提供了依据。Objective To qualitatively analyze the chemical constituents in rats after intragastric administration of estrogenically active ethanol extract of Cuscuta chinensis. Methods The high performance liquid chromatography quadrupole time-of-flight mass spectrometry（HPLC-Q/TOF MS/MS） was used to identify the prototypes and metabolites in rat urine. Results Twelve chemical constituents were identified in the drug-containing urine, including six prototypes and six metabolites. The prototypes are kaemoferol-3-β-D-glucuronide, 6-O-（E）-p-coumaroyl-β-D-fructofuranosyl-（2→1）-α-D-glucopyranoside, aempferol-7-rhamnoside, chlorogenic acid, and apigenin. The metabolites are p-hydroxycinnamic acid, p-hydroxyphenylpropionic acid, isorhamnetin, kaempferol-3-O-glucoside, acetyl caffeic acid, and quercetin sulfate. Conclusion The method of HPLC-Q/TOF MS/MS is simple and rapid for the analysis of prototype components and metabolites in rats urine after oral administration of ethanol extract of C. chinensis, providing the basis for further clarification of the estrogen material basis of C. chinensis.

关 键 词：菟丝子 雌激素作用 HPLC-Q/TOF MS/MS 代谢物 尿液 绿原酸 对羟基桂皮酸 山柰酚-3-O-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]