响应面法优化白花蛇舌草中总黄酮的提取工艺  

Optimization of Extraction Process of Total Flavonoids from Hedyotis diffusa by Response Surface Methodology

作　　者：盛云杰[1] 赵天文[1] 方大宽 陆泽婷 张永生[1] SHENG Yun-jie;ZHAO Tian-wen;FANG Da-kuan;LU Ze-ting;ZHANG Yong-sheng ＊(Pharmacy College,Zhejiang Chinese Medical University,Hangzhou,310053,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州310053

出　　处：《时珍国医国药》2018年第6期1284-1286,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81573700);浙江省自然科学基金(LY16H280004);浙江中医药大学校级科研项目(2018ZZ08)

摘　　要：目的优化白花蛇舌草中总黄酮的提取工艺。方法在单因素试验基础上,以总黄酮为指标,根据Box-Benhnken中心组合设计原理,利用响应面分析法考察料液比、提取浓度、提取时间对白花蛇舌草总黄酮的影响。结果白花蛇舌草中总黄酮提取最佳工艺条件为乙醇浓度90%、料液比1:22、提取时间90min。结论通过响应面法优选的白花蛇舌草总黄酮提取工艺合理可行,可为该药材进一步研究提供实验依据。Objective To optimize extraction process of total flavonoids from He@otis diffusa. Methods On the basis of single factor tests, according to Box - Behnken design principles, response surface analysis was adopted to investigate effects of solid - to - liquid ratio, extraction concentration and time on comprehensive score of extracting amounts of total flavonoids from H. diffusa. Results The optimum extraction conditions of total flavonoids of Heclyotis diffusa were ethanol concentration 90%, ratio of material to liquid 1：22, extraction time 90 min. Conclusion This process is reasonable and feasible with high extraction rate, and it is suitable for industrial production. This study can provide experimental basis for further development and applicationof H. diffusa.

关 键 词：白花蛇舌草 总黄酮 紫外分光光度法 响应面法 

分 类 号：R283[医药卫生—中药学]