刺果番荔枝根提取物对正常小鼠糖耐量的影响及急性毒性研究  

Study on Glucose Tolerance and Acute Toxicity of the Extract of Annona muricata Root in Mice

作　　者：陈永康[1] 张雪映 陈煜 程守前 王烁今 谭银丰[1] 龚晶雯 张小坡[1] 李友宾[1] CHEN Yong-kang;ZHANG Xue-ying;CHEN Yu;CHENG Shou-qian;WANG Shuo-jin;TAN Yin-feng;GONG Jing-wen;ZHANG Xiao-po;LI You-bin ＊(School of Pharmacy Hainan Provincial Key Laboratory of Research and Development of Tropical Herbs,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医学院,海南省热带药用植物研究开发重点实验室,海南海日571199

出　　处：《时珍国医国药》2018年第6期1287-1289,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81460591)

摘　　要：目的研究刺果番荔枝根提取物对正常小鼠糖耐量的影响及小鼠急性毒性。方法将正常小鼠分为模型组,阳性组,提取物高、中、低(50,25,12.5 mg·kg-1)剂量组,静脉注射葡萄糖模型组,静脉注射葡萄糖给药组(25 mg·kg-1),连续口服给药7 d后进行糖耐量测定,观察其对糖耐量的影响。采用改良寇氏法测定提取物半数致死量LD50值及其95%的可信限。结果高、中、低剂量(50,25,12.5 mg·kg-1)提取物均对葡萄糖致正常小鼠高血糖有明显的抑制作用(P<0.01,P<0.01,P<0.05),中剂量(25 mg·kg-1)提取物对静脉注射葡萄糖致正常小鼠高血糖也有显著的抑制作用(P<0.01)。LD50=151.3 mg·kg-1,95%可信限(151.3±18.4)mg·kg-1。结论刺果番荔枝根提取物可显著提高正常小鼠糖耐量。小鼠经口急性毒性属于中等毒性。Objective To study the glucose tolerance and the acute toxicity of the extract of Annona muricata root in normal mice. Methods The glucose tolerance was observed in glucose induced hyperglycemia mouse model. The LD50 and 95% confidence in- terval （CI） was calculated by improved Karber＇s method. Results AGE- III, AGE- II and AGE -I （50, 25, 12.5 mg kg-1 ） decreased the blood glucose in oral glucose load mice （ P 〈 0. 01, P 〈 0. 01, P 〈 0.05 ）. 25 mg kg-l AGE also de- creased the blood sugar in intravenous glucose load mice （P 〈0.01 ）. LD50 of the extract was 151.3mg kg-l （95% CI = 151.3 ＋ 18.4 mg kg-l ）. Conclusion The extract of Annona muricata root can significantly enhance the glucose tolerance of normal mice. Acute toxicity in mice through the mouth belongs to medium poison.

关 键 词：刺果番荔枝根 提取物 血糖 糖耐量 半数致死量 

分 类 号：R285.5[医药卫生—中药学]