基于血清5-HT探讨深刺八髎穴对STC模型大鼠结肠动力的近期与远期效应  

Research about the short-term and long-term effects of deep acupuncture at Baliao points on the colon motility of STC rats based on serum 5-HT

作　　者：金洵[1] 丁曙晴[2] 吕兴 白桦[1] 刘妍[1] JIN Xunj;DING Shu-qing;LIJ Xing;BAI Hua;LIU Yan(Nanjing University of Traditonal Chinese Medine,210023,China;Traditional Chinese Medicine Hospital of Nanjing 210001,China;Central Research Institute of Shanghai Pharmaceuticals Holding Co.,Ltd,Shanghai 200020,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]南京中医药大学第三附属医院,江苏南京210001 [3]上海医药有限公司中央研究院,上海200020

出　　处：《时珍国医国药》2018年第6期1530-1533,共4页Lishizhen Medicine and Materia Medica Research

基　　金：江苏省自然科学基金青年基金项目(BK20161049);江苏省高校自然科学研究面上项目(15KJB360004);国家自然科学基金青年基金项目(81603674)

摘　　要：目的通过观察血清5-HT水平变化,探讨深刺八髎穴改善结肠动力的近期和远期效应规律。方法建立慢传输型便秘大鼠模型,予深刺八髎穴治疗,治疗期第1天、第14天和恢复期第14天,分别检测大鼠体重、血清5-HT水平和肠道推进率等指标。结果治疗期第1天、第14天和恢复期第14天,模型组血清5-HT明显增多(P<0.05),深刺八髎可有效降低其水平(P<0.05)。治疗期第1天和第14天,针刺组血清5-HT显著高于空白组(P<0.05);但恢复期第14天,针刺组和空白组已无差异。说明针刺1次,深刺八髎的效应已经呈现;14次治疗后,针刺耐受尚未出现;停止针刺14天后,还能表现显著的后效应。肠道推进率结果与5-HT水平变化不完全吻合,治疗过程中针刺组肠道推进率的改善并不明显,但也可观察到针刺后效应。结论深刺八髎可下调STC大鼠血清5-HT水平,并随时间变化呈现一定规律,能观察到明显的效应期和后效应期,这可能是深刺八髎治疗STC的重要机制之一,血清5-HT水平可用作效应指标。但仍需进一步观察深刺八髎穴效应潜伏期及各期的持续时间,这对临床针灸效应的维持和积累、确定疗程具有指导意义。Abstract： Objective To research the short - term and long - term effects of deep acupuncture at Baliao points on the colon mo- tility by observing changes in serum 5 - HT levels. Methods The STC rat model was established and deep acupuncture was opera- ted at Baliao points. The body weight, serum 5 -HT level and Intestinal transit rate were measured on the first day, the 14th day of treatment period and the 14th day of recovery period. Results Serum levels of 5 - HT in the model group increased significantly（P 〈 0.05 ） on the first day, the 14th day of treatment period and the 14th day of recovery period, which could be reduced by deep acupuncture at Baliao points （ P 〈 0.05 ）. The levels of serum 5 - HT in the acupuncture group were significantly higher than those in the normal group （P 〈 0.05 ） on the first day, the 14th day of treatment period. On the 14th day of recovery period, there was no difference between the acupuncture group and the normal group. The effect of acupuncture has been presented after 1 time treatment. Acupuncture tolerance has not appeared after 14 times treatment. Stop acupuncture 14 days later, a significant post - effect could be observed. The results of Intestinal transit rate were not consistent with the changes of 5 - HT level. The im- provement of Intestinal transit rate in acupuncture group was not obvious improved during treatment, but the post - effect of acu- puncture was also observed. Conclusion The level of serum 5 - HT in STC rats can be down - regulated by deep acupuncture at Baliao points with obvious effect and post - effect period, which may be an important mechanism for the treatment of STC on Baliao points. Serum 5 - HT levels can be used as effectors. However, it is necessary to further observe the latency and the dura- tion of each period, which could guide the maintenance and accumulation of acupuncture effect.

关 键 词：深刺 八髎 5-HT 结肠动力 效应规律 

分 类 号：R284.2[医药卫生—中药学]