银屑Ⅰ号抑制TNF-α诱导Hacat细胞炎症反应的机制研究  

Mechanism of Yinxie Ⅰ Hao Decoction for Inhibiting Inflammatory Response of Hacat Cell Model Induced by Tumor Necrosis Factor α

作　　者：孙文 余先华[1] 彭星[1] 高扬[2] 刘靖[2] 查旭山[2] SUN WEN;YU Xianhua;YUAN YUAN;GAO YANG;LIU JING;CHA Xushan(Department of Dermatology,The First People＇s Hospital of Jingmen,Jingmen 448000,Hubei,China;Department of Dermatology,The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong,China)

机构地区：[1]荆门市第一人民医院皮肤科,湖北荆门448000 [2]广州中医药大学第一附属医院皮肤科,广东广州510405

出　　处：《辽宁中医杂志》2018年第7期1511-1513,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81573980);国家自然科学基金青年项目(81202699);湖北省自然科学基金青年项目(2018CFB289)

摘　　要：目的：观察银屑I号对肿瘤坏死因子α（TNF-α）诱导的人永生化角质形成细胞（Hacat）炎症反应的影响并探讨其机制。方法：采用低、中、高浓度的银屑I号含药血清干预Hacat细胞,ELISA法检测细胞上清液25HVD3、IL-1、IL-17、IL-22浓度,Western-blot法检测维生素D受体（vitamine D receptor,VDR）蛋白表达量,RT-PCR检测VDRmRNA相对表达量。结果：与空白组比较,模型组VDR蛋白、VDRmRNA、25HVD3表达量明显降低,IL-1、IL-17、IL-22明显升高（P〈0.01）;与模型组比较,银屑I号各组VDR蛋白、VDRmRNA、25HVD3表达量明显升高,IL-1、IL-17、IL-22明显降低（P〈0.05）,且呈剂量依赖性。讨论：银屑I号可能通过促进VDR蛋白、VDRmRNA及25HVD3因子信号表达发挥对银屑病的治疗作用。Objective：To investigate the impact of Yinxie I Hao Decoction on the inflammatory response of Hacat cell model induced by tumor necrosis factor α and discuss its mechanism. Methods：Hacat cells were cultured by low,medium and high concentrations of Yinxie I Hao Decoction drug-containing serum,respectively. Enzyme-linked immunosorption test was inducted to detect the concentrations of 25 HVD3,IL-1,IL-17,IL-22 of supernatant fluid. The Western-blot was inducted to detect the expression of vitamin D receptor and the reverse transcription-polymerase chain reaction was inducted to detect the relative expression of VDRmRNA. Results：Compared with the blank group,the expressions of VDR protein,VDRmRNA and 25 HVD3 were decreased significantly and the concentrations of IL-1,IL-17 and IL-22 were increased obviously in model group（ P〈0.01）.Compared with the model group,the expressions of VDR protein,VDRmRNA and 25 HVD3 were increased significantly,while the concentrations of IL-1,IL-17 and IL-22 were decreased obviously in each dose groups of Yinxie I Hao（ P〈0.05）,and the influence was associated with concentration. Conclusion：Yinxie I Hao Decoction could improve the expressions of VDR protein,VDRmRNA and 25 HVD3 to antagonize inflammatory reaction.

关 键 词：银屑Ⅰ号 维生素D受体 人永生化角质形成细胞 白介素-22 炎症反应 

分 类 号：R285.5[医药卫生—中药学]