基于TFF3、ERK/NF-κB信号通路探讨加味七方胃痛颗粒对人胃癌细胞株SGC7901增殖凋亡的影响  

Effect of Jiawei Qifang Weitong Granules on Proliferation and Apoptosis of Human SGC-7901 Cells Based on TFF3 and ERK/NF-κB

作　　者：黄丽萍[1] 陈国忠[2] 刘锟荣 谢卓恩 苏洪佳 曾丽金[1] 李兵[1] 彭鸿 方丽娇 彭振西 HUANG Liping;CHEN Guozhong;LIU Kunrong;XIE Zhuoen;SU Hongjia;ZENG Lijin;LI Bing;PENG Hong;FANG Lijiao;PENG Zhengxi(Postgraduate School,Guangxi University of TCM,Nanning 530001,Guangxi,China;The First Affiliated Hospital of Guangxi Univeristy of TCM,Nanning 530023,Guangxi,China)

机构地区：[1]广西中医药大学研究生院,广西南宁530001 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《辽宁中医杂志》2018年第7期1516-1521,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81260529,81560758)

摘　　要：目的：探讨加味七方胃痛颗粒对人胃癌细胞株SGC7901肠三叶因子3（Trefoil Factor3,TFF3）的表达及其细胞外信号调节激酶（extracellular signal-regulated kinase,ERK）/核因子κB（nuclear factor-κB,NF-κB）信号通路的调控机制。方法：参照血清药理学方法制备加味七方胃痛颗粒含药血清,分别用不同剂量的含药血清、5-氟尿嘧啶（5-FU）和生理盐水对SGC 7901进行培养。分别采用四甲基偶氮唑蓝（MTT）法及流式细胞术检测细胞增殖及凋亡;采用western blot、RT-PCR检测细胞TFF3、NF-κB、ERK mRNA及蛋白表达水平。结果：与空白对照组相比,加味七方胃痛颗粒能抑制SGC 7901的增殖、促进其凋亡（P〈0.05）,并呈浓度、时间依赖性;中药血清干预后人胃癌细胞株TFF3、ERK、NF-κB蛋白和mRNA表达降低,与空白对照组相比差异具有统计学意义（P〈0.05）。结论：在体外试验中,加味七方胃痛颗粒能抑制SGC 7901的增殖并促进其凋亡,可能通过下调胃癌细胞TFF3、ERK/NF-κB蛋白的表达水平,从而到达抗癌作用。Objective：To investigate the effects of serum containing Chinese medicine（ CM） Jiawei Qifang Weitong（ JWQFWT） Granules on the proliferation and apoptosis of human SGC-7901 cells and the possible mechanism. Methods：Serum containing JWQFWT Granules（ JWQFWT serum） was prepared by a serum pharmacology method. Human SGC-7901 cells were cultivated with JWQFWT serum at three different concentrations,the anticancer drug 5-fluorouracil（5-FU） and the normal salina,respectively. Cell proliferation was assessed by MTT assay,and cell apoptosis was detected byflow cytometry assay. Real-time quantitative polymerase chain reaction（ RT-PCR） and Western blot assay were employed to confirm the expressions of TFF3,NF-κB and ERK in SGC-7901 cells at mRNA and protein levels,respectively. Results：MTT assay showed that compared to the blank group,JWQFWT serum restrained the proliferation of SGC-7901（ P〈0.05）,and the inhibition rate increased significantly in a time and dose-dependent manner. RT-PCR and western blot assay indicated that JWQFWT serum down-regulated the protein and mRNA expression levels of TFF3,NF-κB and ERK. Compared with the blank group,there was a statistical significance difference（ P〈0.05）. Conclusion：JWQFWT Granules inhibit the proliferation of SGC-7901 cells in vitro and induces the cell apoptosis. It plays an anticancer role by regulating the expressions of TFF3,NF-κB and ERK in SGC-7901 cells.

关 键 词：胃癌 中药 信号通路 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]