血小板冻干过程对其生长因子释放量和促HUVECs增殖作用影响的实验研究  

An experimental study on the growth factor contents release and HUVECs proliferation in platelets treated with lyophilization

作　　者：周谋[1] 唐艳姣 施琳颖[1] 魏淑贞 林放[1] 李文丹[1] 单桂秋[1] ZHOU Mou;TANG Yanjiao;SHI Linying;WE;LIN Fang;LI Wendan;SHAN Guiqiu(Guan-gzhou General Hospital of Guangzhou Military Command,Guangzhou 510010,China;The Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People＇s Hospital,Qingyuan 510010,China;Guangzhou University of Chinese Medicine,Guangzhou 510405,China.)

机构地区：[1]广州军区广州总医院,广东广州510010 [2]广州医科大学附属第六医院清远市人民医院 [3]广州中医药大学

出　　处：《中国输血杂志》2018年第6期593-596,共4页Chinese Journal of Blood Transfusion

基　　金：军队后勤重点科研项目《急救输血器材和血液制品关键技术研究》(BGZ15C002);广州市科技计划项目《新型可溶性血小板凝胶纱布的研制》(201508020115)

摘　　要：目的探讨血小板冻干过程对其释放PDGF-BB、TGF-β1、VEGF、EGF、IGF-I和FGF-b 6种生长因子及其促HUVECs增殖作用的影响。方法采用ELISA法检测冻干前新鲜PRP激活上清液和FDP激活上清液中6种生长因子的含量。用含不同浓度的FDP激活上清液、新鲜PRP激活上清液和FBS的培养基分别培养HUVECs,CCK8法检测HUVECs增殖的状态。结果 FDP上清液中TGF-β1、VEGF、EGF、IGF-I和FGF-b含量均高于新鲜PRP激活上清液,差异均有统计学意义（P〈0.05）;而PDGF-BB含量略低于新鲜PRP激活上清液,差异无统计学意义（P〉0.05）。FDP激活上清液、PRP激活上清液和FBS均可促进HUVECs的增殖;随着FDP和PRP激活上清液浓度的增高,细胞增殖作用反而降低,差异有统计学意义（P〈0.05）;在5%的浓度条件下,培养48 h,FDP激活上清液促进HUVECs增殖作用高于PRP激活上清液和FBS,差异有统计学意义（P〈0.05）;培养24 h和72 h,FDP激活上清液、PRP激活上清液和FBS促进HUVECs增殖作用无统计学差异（P〉0.05）。结论 FDP具有优于新鲜PRP释放生长因子的能力;FDP激活上清液促HUVECs增殖作用优于PRP激活上清液,与FBS具有可比性。Objective To investigate the impact of lyophilization on growth factor release and HUVEC proliferation in platelets. Methods The contents of 6 growth factors in fresh PRP-activated supernatant and FDP-activated supernatant were measured by ELISA. HUVECs were cultured with different concentrations of FDP-activated supernatant,fresh PRP-activated supernatant and FBS. The proliferation status of HUVECs was evaluated by CCK8. Results The concentration of PDGF-BB in activated supernatant of FDP is slightly lower than that in PRP-activated supernatant with no significant differences（ P〈0. 05）. The levels of other 5 growth factors,TGF-β1,VEGF,EGF,IGF-I and FGF-b,in the activated supernatant of FDP were significantly higher than that in PRP-activated supernatant（ P〈0. 05）. The activated supernatants of FDP,PRP and FBS promoted the proliferation of HUVECs. With the increase of FDP and PRP-activation supernatant concentration,cell proliferation activity decreased with a significant differences（ P〈0. 05）. At a concentration of 5%,a statistically significant increase in HUVECs cultured in the presence of FDP-activated supernatants was observed at 48 hours in comparison to the PRP-activated supernatants and FBS. However,from 48 to 72 hours,the proliferation of HUVECs did not differ significantly among these three groups. Conclusion FDP helped to release growth factors better than fresh PRP. The FDP activated-supernatant is superior to the PRP-activated supernatant in terms of HUVECs proliferation,and it is comparable with FBS.

关 键 词：冻干血小板 PRP 生长因子 细胞增殖 

分 类 号：R446.6[医药卫生—诊断学] R331.1[医药卫生—临床医学]