不同浓度葡萄糖、氯化钠及氯化铁对铜绿假单胞菌生物膜形成的影响研究  

Study on the Effects of Different Concentration Glucose,Sodium Chloride,Ferric Chloride on the Biofilm of Pseudomonas Aeruginosa

作　　者：李瑜珍[1] 郑琳 张惠媚[1] 张春雷[1] LI Yu-zhen a;ZHENG Lin b;ZHANG Hui-mei a;ZHANG Chun-lei a(a.Department of Clinical Laborator;b.Key Laboratory of Traditional Chinese Medicine Research,Shenzhen TCM Hospital,Guangdong Shenzhen 518034,China)

机构地区：[1]深圳市中医院检验科,广东深圳518034 [2]深圳市中医院中药制剂研究重点实验室,广东深圳518034

出　　处：《现代检验医学杂志》2018年第4期131-133,共3页Journal of Modern Laboratory Medicine

基　　金：深圳市科技计划项目(JCYJ20150401163247217);广东省医学科学基金项目(A2017402)

摘　　要：目的研究不同浓度葡萄糖、氯化钠及氯化铁对铜绿假单胞菌生物膜形成的影响,为铜绿假单胞菌生物膜形成的预防和治疗提供新的思路,及为其体外生物膜模型的构建提供实验优化条件。方法 LB培养基中加入不同浓度葡萄糖、氯化钠、氯化铁,使葡萄糖浓度分别为0,27.75,55.51,83.26和111.01 mmol/L;氯化钠浓度分别为0,85.56,171.12,256.67和342.23mmol/L;氯化铁浓度分别为0.77,1.54,3.09和6.17mmol/L,与铜绿假单胞菌共培养,以不加葡萄糖、氯化钠及氯化铁为对照组,用结晶紫染色法半定量检测生物膜生成的量。结果葡萄糖55.51mmol/L组,氯化钠85.56mmol/L及171.12 mmol/L组,氯化铁3.09 mmol/L组生物膜生成量与对照组比较,差异有统计学意义(P=0.020,0.036,0.017,0.041)。结论 55.51mmol/L葡萄糖,85.56mmol/L及171.12mmol/L氯化钠,3.09mmol/L氯化铁会促进铜绿假单胞菌生物膜生成,糖尿病患者感染铜绿假单胞菌应注意控制血糖浓度,铜绿假单胞菌感染的患者不要输注氯化钠溶液,伤口避免用氯化钠溶液清洗,预防生物膜的生成。Objective To study the effect of different concentrations glucose,sodium chloride,ferric chloride on the biofilm producing capacity of Pseudomonas aeruginosa,and provide new strategy for the P.aeruginosabiofilm prevention/treatment and optimization of the condition of P.aeruginosa biofilm experiment model in vitro.Methods P.aeruginosa were co-cultured with glucose at the concentration of 0,27.75,55.51,83.26 and 111.01 mmol/L,with NaCl at the concentration of 0,85.56,171.12,256.67 and 342.23 mmol/L,and with FeCl_3 at the concentration of 0,0.77,1.54,3.09 and 6.17 mmol/L respectively.Compared with the control groups with no glucose,NaCl or FeCl_3,the biofilm producing capacity of P.aeruginosa were tested by semi-quantitative crystal violet method in vitro.Results The groups of 55.51 mmol/L glucose,85.56 mmol/L,171.12 mmol/L sodim chloride and 3.09 mmol/L FeCl_3 was respectively compared with the control group the differences were statistically significant（P=0.020,0.036,0.017,0.041）.Conclusion 55.51 mmol/L glucose,85.56 mmol/L NaCl,171.12 mmol/L NaCl,3.09 mmol/L FeCl_3 treatment could significantly induce the biofilm producing of P.aeruginosa.To prevent the P.aeruginosa biofilm producing,patient infected with P.aeruginosashould control blood glucose and reduce sodium intake.

关 键 词：葡萄糖 氯化钠 氯化铁 铜绿假单胞菌生物膜 

分 类 号：R378.991[医药卫生—病原生物学] R446.5[医药卫生—基础医学]