蔗糖,GA与液体培养基对马铃薯块茎形成的影响  

Effect of Sucrose,GA and Liquid Medium on the Tuber Formation of Potato

作　　者：何依雪 刘文 沈祥陵 HE Yi-xue;LIU Wen;SHEN Xiang-ling(Key Laboratory of Three Gorges Regional Plant Genetics ＆ Germplasm Enhancement（CTGU）/Hubei Engineering Research Center for Three Gorges Regional Plant Breeding/Biotechnology Research Center,China Three Gorges University,Yichang 44300)

机构地区：[1]三峡区域植物遗传与种质创新重点实验室(三峡大学)湖北省三峡特色植物繁育工程技术研究中心三峡大学生物技术研究中心,宜昌443000

出　　处：《生物技术通报》2018年第7期74-80,共7页Biotechnology Bulletin

基　　金：三峡大学高层次人才科研启动基金

摘　　要：马铃薯是全球第四大粮食作物,粮菜兼用,有丰富的营养价值。马铃薯块茎是马铃薯最具经济价值的部分。马铃薯块茎形成的过程受多种调控途径的调控,其中最重要的是蔗糖途径和赤霉素(GA)途径。本实验设计蔗糖浓度,GA浓度和培养基类型3个因素的正交试验,通过对马铃薯的结薯时间,结薯数,马铃薯均重和大薯率的统计发现高浓度的蔗糖能够使马铃薯结薯提前,液体培养基能够增加马铃薯的薯重和大薯率。添加8%蔗糖和0.1 mg/L GA的液体MS培养基能够使马铃薯提前结薯并且得到的马铃薯数量多,薯重大。Potato is the fourth largest food crop in the world. It has been used for both grain and vegetable and is rich in nutritional value.Potato tubers are the most economically valuable part of the potato. The formation process of potato tuber is regulated by a variety of regulatorypathways,the most important ones of which is the sucrose pathway and the gibberellin（GA）pathway. This experiment was designed toconduct orthogonal testing three factors of sucrose concentration,GA concentration and media type. By the statistics of potato tuber formationtime,the number of potato tubers,potato average weight,and the rate of large potato,it was found that high concentration of sucroseadvanced potato tubering,and liquid medium increased the potato weight and the rate of large potato. The addition of 8% sucrose and 0.1 mg/LGA in liquid MS medium resulted in advanced potato tuber formation as well as more and heavier potatoes.

关 键 词：蔗糖 赤霉素 液体培养基 马铃薯结薯 

分 类 号：S532[农业科学—作物学]