白细胞介素13对人鼻息肉黏膜上皮细胞嗜酸性趋化因子3蛋白含量及mRNA影响的实验研究  

Experimental study on the effect of TH2 cell related factor IL-13 on CCL26 protein content and mRNA in human epithelial cells of nasal polyps

作　　者：欧华霜 田鹏[1] 邹华[1] 吴帆[1] 马赟[1] 刘翔[1] 党华 陈秋坚[1] OU Huashuang;TIAN Peng;ZOU Hua;WU Fan;MA Yun;LIU Xiang;DANG Hua;CHEN Qiujian(Department of Otolaryngology Head and Neck Surgery,Sun Yat-sen Memorial Hospital,Sun Yat-Sen University Guangzhou,Guangdong,510120,China)

机构地区：[1]中山大学孙逸仙纪念医院耳鼻咽喉头颈外科,广东广州510120

出　　处：《中国耳鼻咽喉头颈外科》2018年第8期449-452,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金项目(81500773);广东省自然科学基金项目(2015A030310125)联合资助

摘　　要：目的观察白细胞介素13(interleukin-13,IL-13)刺激鼻息肉黏膜上皮细胞后嗜酸性趋化因子3,即CCL26蛋白含量及其mRNA表达变化,探讨嗜酸性粒细胞浸润鼻黏膜的机制。方法以IL-13细胞因子刺激原代培养的人嗜酸性鼻息肉黏膜上皮细胞。用实时定量PCR法检测CCL26 mRNA的表达,ELISA检测CCL26蛋白表达。结果与对照组相比,IL-13刺激组鼻息肉黏膜上皮细胞CCL26蛋白分泌增加,差异具有统计学意义(P<0.05)。其增高呈浓度依赖性。结论 IL-13可诱导鼻息肉黏膜上皮细胞分泌CCL26蛋白。OBJECTIVE To invest igate the changes of CCL26 protein content and its mRNA expression after stimulation of nasal polyps derived epithelial cells by Th2 cytokines, and to explore the mechanism of eosinophil inf iltration on nasal mucosa. METHODS Primar y cultured human eosinophilic nasal polyps derived nasal epithelial cells were stimulated with IL-13 cytokines. The expression of CCL26 mRNA was detected by real timePCR and the expression of CCL26 protein was detected by enzyme-linked immunosorbent assay（ELISA）. RESULTS Compared with the control group, IL-13 stimulated the secretion of CCL26 protein from nasal epithelial cells. The difference was statistically significant（P〈 0.05, n=3）. The increase is concentration-dependent. CONCLUSION Cytokine IL-13 can induce the secretion of CCL26 protein in nasal mucosal epithelial cells from nasal polyps.

关 键 词：白细胞介素13 趋化因子 CC 鼻息肉 上皮细胞 趋化因子CCL26 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]