竹节参水煎液对AD大鼠海马神经元尼氏体的影响  

Effects of Panax japonicus Decoction on Nissl Bodies of Hippocampal Neurons in AD Rats

作　　者：曾楚华 王文晟 鲁文杰 袁德培 陈玮 李瑞 牟南樵 Zeng Chuhua;Wang Wensheng;Lu Wenjie;Yuan Depei;Chen Wei;Li Rui;Mou Nanqiao(Medical College of Hubei University for Nationalities,Enshi,44500)

机构地区：[1]湖北民族学院医学院

出　　处：《基因组学与应用生物学》2018年第9期4030-4035,共6页Genomics and Applied Biology

基　　金：国家自然科学基金项目(81260521);湖北民族学院(MY2015B049)共同资助

摘　　要：探讨竹节参对AD大鼠的作用及海马神经元尼氏体的影响。60只SPF级SD雄性大鼠,随机分为6组,假手术组海马注射生理盐水,其余均注射Aβ（1-42）以复制AD模型。假手术组及模型组予以生理盐水灌胃,西药组予脑复康悬浮液灌胃,竹节参高、中、低剂量组分别予相应浓度竹节参水煎液灌胃,持续4 w。灌胃结束后,采用Morris水迷宫进行定位航行实验和空间探索实验。取大鼠海马切片,尼氏染色法观察海马神经元尼氏体。定位航行试验中,各组逃避潜伏期均呈逐日下降趋势,但模型组下降趋势最缓,尤其明显缓于假手术组,而西药组和竹节参高、中、低剂量组下降趋势均快于模型组。空间探索试验中,模型组第一次穿越平台的时间明显长于假手术组（p〈0.001）,各治疗组明显短于模型组,且竹节参高剂量组短于西药组;穿越平台的次数,模型组明显少于假手术组（p〈0.001）,各治疗组明显多于模型组,且竹节参高剂量组多于西药组。尼氏染色法观察到模型组大鼠海马神经元的尼氏体明显少于假手术组（p〈0.001）,各治疗组明显多于模型组,且竹节参高剂量组要优于西药组。竹节参能改善海马神经元形态和数量,从而提高AD模型大鼠的学习记忆能力。To investigate the effects of Panax japon icus on AD rats and the effect of Nissl bodies on those hippocampal neurons. 60 male SD rats were randomly divided into six groups： sham operation group, model group, western medicine group, low dosage group of Panax japonicus decoction, middle dosage group and high dosage group. In addition to sham operation group injection of normal saline, the other five groups were injected with Aβ（1-42） into bilateral hippocampus to produce AD model. The rats in the sham operation group and the model group were treated with normal saline orally. The rats in the western medicine group were treated with piracetam suspension and the low, middle and high dosage groups were treated with Panax japonicus decoction by gavage.The time of gavage in each group was four weeks. Morris water maze was used to observe the effects of Panax japonicus on the spatial learning and memory ability of rats in each group when the gavage was over.The distribution and development of neurons in the hippocampus were observed by Nissl staining. In the navigation test, the escape trend of each group showed a decreasing trend, but the model group showed the slowest trend,especially in the sham operation group, while the descending trend of the western medicine group and the Panax japonicus group was higher than that of the model group. In space exploration experiments, compared with the sham operation group, the first time of crossing the platform in the model group was longer（p〈0.001）, treatment groups were shorter than model group, and the time of the high dose group was shorter than that of the western medicine group. For the frequency of crossing the platform, the model group was significantly less than the sham operation group（p〈0.001）, the treatment group was significantly more than the model group and the high dose group was more than western medicine group. It was observed by Nissl＇s staining that the Nissl bodies of hippocampal neurons in model group were significantly less than those

关 键 词：竹节参 阿尔兹海默病 AΒ1-42 学习记忆 神经元尼氏体 

分 类 号：R285.5[医药卫生—中药学]