乙烯对‘班克海尔’和‘杨妃出浴’芍药切花开放和衰老的影响  

Effects of Ethylene on Opening and Senescence Process of Herbaceous Peony Cut Flowers 'Banker Hill' and 'Yangfei Chuyu'

作　　者：杨若雯[1] 张萍[1] 薛玉前 薛璟祺[1] 王顺利[1] 张秀新[1] YANG Ruowen;ZHANG Ping;XUE Yuqian;XUE Jingqi;WANG Shunli;ZHANG Xiuxin(Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,P.R.China,Institute of Peony,Chinese Academy of Agricultural Sciences,National Center for Flower Improvement,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所国家花卉改良中心中国农业科学院牡丹研究中心农业部园艺作物生物学与种质创制重点实验室,北京100081

出　　处：《园艺学报》2018年第8期1575-1586,共12页Acta Horticulturae Sinica

基　　金：中国农业科学院基本科研业务费专项(IVF-BRF2018019);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);中国科协青年人才托举工程项目(2015QNRC001)

摘　　要：以‘班克海尔’和‘杨妃出浴’芍药切花为试材,研究了乙烯利处理对切花开放和衰老的影响;同时从中克隆得到乙烯生物合成和信号转导的9个关键基因PlACS、PlACO、PlACO5、PlETR1、PlERS1、PlETR2、PlEIN4、PlCTR1和PlEIN3,利用qRT-PCR分析这些基因的表达模式。结果表明:‘班克海尔’正常瓶插寿命长于‘杨妃出浴’,两品种均对乙烯敏感;乙烯利明显促进‘班克海尔’花朵开放和衰老进程,抑制‘杨妃出浴’花朵开放进程,并且出现僵花、衰老萎蔫。基因序列分析表明,乙烯合成和信号转导基因在两种切花品种中高度保守,保守性在98%以上。qRT-PCR分析表明,瓶插12~36 h,乙烯诱导两品种中除PlEIN3外的所有基因上调。瓶插0~12 h,‘杨妃出浴’中9个基因表达量均较高,内源乙烯合成和乙烯信号转导较为活跃,而在24~36 h,外源乙烯使‘班克海尔’乙烯生物合成基因表达量升高,乙烯信号转导基因表达量普遍高于‘杨妃出浴’。推测‘杨妃出浴’自身合成乙烯的时间较早,对乙烯的反应比‘班克海尔’更为敏感,‘班克海尔’乙烯反应类型可能为末期上升型,而‘杨妃出浴’为乙烯跃变型;两种芍药切花对乙烯的敏感程度以及花朵开放和衰老进程不同,可能与其乙烯生物合成和信号转导相关基因表达模式不同有关。Herbaceous peony cut flowers of＇Banker Hill＇and＇Yangfei Chuyu＇were used to investigate the effects of ethrel on flower opening and senescence processes. Nine ethylene biosynthesisand signal transduction-genes,including PlACS,PlACO,PlACO5,PlETR1,PlERS1,PlETR2,PlEIN4,PlCTR1 and PlEIN3 were cloned from the two cultivars. The expression patterns of the nine genes were analyzed by qRT-PCR. The results showed that＇Banker Hill＇had longer vase life than＇Yangfei Chuyu＇under normal conditions,and both cultivars were sensitive to ethylene. Ethrel treatment inhibited flower opening severely,and resulted in bull flower and even wilting directly in＇Yangfei Chuyu＇,while it accelerated the processes of flowering opening and senescence in＇Banker Hill＇. Sequence alignments of nine genes showed that they were highly conserved in the two cultivars,and the sequence identity was above 98%. The q RT-PCR results showed that ethrel treatment could increase all the above gene expression level except PlEIN3 in two cultivars,in 12–36 h. In 0–12 h,the nine genes had higher expression level in＇Yangfei Chuyu＇than in＇Banker Hill＇,and it was deduced that the endogenous ethylene synthesis and signal transduction were more active in＇Yangfei Chuyu＇,while in 24–36 h,ethrel treatment promoted the ethylene synthesis-and signal transduction-genes expression in＇Banker Hill＇,and the ethylene signal transduction-genes expression levels in＇Banker Hill＇were higher than those in＇Yangfei Chuyu＇. The delayed ethylene responses could promote flower quality of＇Banker Hill＇. Therefore,it was suggested that＇Yangfei Chuyu＇is more sensitive to ethylene than＇Banker Hill＇. Differences of expression levels of ethylene biosynthesis-and signal transduction-genes may cause different sensitivity type to ethylene of the two cut flowers,and affect flower opening and senescence process.

关 键 词：芍药 切花 乙烯利 乙烯合成和信号转导基因 基因表达 

分 类 号：S682.12[农业科学—观赏园艺]