建兰花发育相关B、C和E类MADS-box基因的表达分析  

Expression Analysis of B,C and E Class MADS-box Genes in Cymbidium ensifolium

作　　者：向林[1] 陈跃[2] 陈丽萍[2] 孙崇波[2,3] XIANG Lin;CHEN Yue;CHEN Liping;SUN Chongbo(Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;1nstitute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Key Laboratory of Creative Agriculture,Ministry of Agriculture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]华中农业大学园艺林学学院园艺植物生物学教育部重点实验室,武汉430070 [2]浙江省农业科学院园艺研究所,杭州310021 [3]浙江省农业科学院农业部创意农业重点实验室,杭州310021

出　　处：《园艺学报》2018年第8期1595-1604,共10页Acta Horticulturae Sinica

基　　金：浙江省农业新品种选育重大科技专项(2016C02056-13);中央高校基本科研业务费专项资金项目(2662016QD016);浙江省农业科学院人才项目(2016R05R08E03);浙江省自然科学基金项目(LQ17C150002)

摘　　要：开花植物中B、C和E类MADS-box基因控制花雄蕊和雌蕊的合成。以具有高度进化蕊柱(雄蕊和雌蕊合生)的建兰为材料,从已有转录组中搜索到14个B、C和E类基因,包括GLO、DEF、AG、SEP和AGL6类基因,并进行实时荧光定量表达分析,发现CeDEF1和CeAGL6-1在萼片和花瓣中大量表达,唇瓣中微量表达;CeDEF3、CeDEF4和CeAGL6-3则在唇瓣和蕊柱中大量表达,在萼片和花瓣中几乎不表达;CeAG1和CeAG2主要在蕊柱中表达;而且CeDEF3、CeDEF4、CeAG1、CeAG2和CeAGL6-3在蕊柱突变体‘新品梅’的蕊柱状花瓣中的表达量比‘铁骨素’花瓣中高很多,说明这几个基因在建兰蕊柱的形成过程中可能扮演关键角色。Cymbidium ensifolium（L.）flower is highly evolved with a column（a fusion of the male and female reproductive organs）. Its complex floral architecture presents an exciting opportunity to examine column development. In flowering plants,class B,C and E floral homeotic genes play key roles in the specification of stamen and carpel identity. In this study,Fourteen class B,C and E unigenes（including DEF-,GLO-,AG-,SEP-,and AGL6-like unigenes）were detected from transcriptome data of C. ensifolium flower buds. Real-time quantitative PCR results showed that CeDEF1 and CeAGL6-1 were only strongly detected in the sepals and petals,and were significantly down-regulated in the lips. CeDEF3,CeDEF4 and CeAGL6-3 were expressed highly in the lips and columns,but were detected minimally in the sepals and lateral petals. CeAG1 and CeAG2 were strongly expressed in columns. In addition,the expression levels of CeDEF3,CeDEF4,CeAG1,CeAG2 and CeAGL6-3 were higher in column-like petals of＇Xinpinmei＇than in petals of＇Tiegusu＇. These results displayed that CeDEF3,CeDEF4,CeAG1,CeAG2 and CeGL6-3 may play key roles in regulating the column formation of C. ensifolium.

关 键 词：建兰 MADS-BOX基因 蕊柱 发育 实时荧光定量表达 

分 类 号：S682.31[农业科学—观赏园艺]