干燥综合征患者唇腺浆细胞分布特点及相关因素的研究  

Distribution characteristics of plasma cells in labial glands of patients with Sj?gren's syndrome

作　　者：刘军[1] 郭娟 周炜[1] Liu Jun;Guo Juan;Zhou Wei(Department of Rheumatology and Immunology,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院风湿免疫科,100034

出　　处：《中国医药》2018年第9期1377-1381,共5页China Medicine

基　　金：国家自然科学基金(81373196)~~

摘　　要：目的研究干燥综合征(SS)患者唇腺浆细胞分布特点及相关因素。方法收集2013年7月至2016年12月52例在北京大学第一医院风湿免疫科行唇腺活检患者(SS患者28例,非SS患者22例)的临床资料,记录患者抗核抗体谱和免疫球蛋白(Ig)水平,取其留存石蜡包埋唇腺组织再次切片进行CD138免疫组织化学染色,计数CD138+浆细胞数量。比较SS患者与非SS患者浆细胞数量差别,分析SS患者浆细胞数量与Ig水平的相关性。结果 SS患者与非SS患者唇腺的间质中均有散在浆细胞,并有数个浆细胞聚集的现象,而SS患者唇腺间质浆细胞数量明显多于非SS患者[(1 263±1 051)个/mm^2比(562±360)个/mm^2],差异有统计学意义(P=0.002)。SS患者唇腺中浆细胞在淋巴细胞浸润灶内的分布有很大差异,由零星少量浆细胞,到较多浆细胞并有浆细胞聚集现象,再到大量浆细胞聚集成灶。SS唇腺浆细胞聚集灶常出现在大的淋巴细胞聚集灶内及其周边。SS患者唇腺间质内和淋巴细胞灶内的CD138^+浆细胞总数与血清IgG和IgA水平均无相关性(r=0.22、P=0.26,r=-0.14、P=0.50)。结论 SS患者唇腺组织中浆细胞明显增多,可以出现浆细胞聚集灶。而SS浆细胞聚集灶的形成与唇腺大的淋巴细胞浸润灶相关。Objective To investigate the distribution characteristics of plasma cells in labial glands of Sjogren＇s syndrome （SS）.Methods From July 2013 to December 2016,clinical data of 28 SS patients and 24 non-SS patients who had labial gland biopsy in Department of Rheumatology and Immunology,Peking University First Hospital were reviewed,including the records of anti-nuclear antibody and immunoglobulin （Ig）.Parafin section of labial glands was tested by immunohistochemistry and the number of CD138 ＋ plasma cells was counted.Relation between CD138 ＋ plasma cell number and Ig level was analyzed.Results CD138 ＋ plasma cells presented in interstitial tissue of labial glands in both SS and non-SS patients;the number of CD138＋ plasma cells in SS patients was significantly higher than that in non-SS patients[（1 263 ± 1 051） cells/mm^2 vs （562 ± 360）cells/mm^2]（P =0.002）.In SS patients,the distribution of plasma cells in infiltrating lymphocytic foci presented significant differences among different individuals;some were scattering;some cells aggregated;some were plasma cell aggregation foci.Most plasma cell aggregation foci were near the large lymphocyte foci.The total number of CD138 ± plasma cells in interstitial tissue and lymphocyte foci showed no significant correlation with serum levels of IgG and IgA （r =0.22,P =0.26;r =-0.14,P =0.50）.Conclusion The number of plasma cells increases in labial glands in SS patients;some patients have plasma cell aggregation foci,which may be related to the large lymphocytic infiltrating foci.

关 键 词：干燥综合征 淋巴细胞 浆细胞 CD138 唇腺 

分 类 号：R593.2[医药卫生—内科学]