SIRT1对高糖环境下血管内皮细胞活力及ROS水平的影响  

Effects of SIRT1 on the vascular endothelial cell viability and ROS level in high glucose environment

作　　者：王科峰[1] 柴林燕[2] 张允东 高海燕[1] WANG Ke-feng;CHAI Lin-yan;ZHANG Yun-dong;GAO Hai-yan(Department of Endocrinology,the Second Affiliated Hospital of Xi＇an Medical University,Xi＇an 710038,China)

机构地区：[1]西安医学院第二附属医院内分泌科,陕西西安710038 [2]西安交通大学第一附属医院肿瘤放疗科,陕西西安710061 [3]西安医学院第二附属医院外科,陕西西安710038

出　　处：《武警后勤学院学报（医学版）》2018年第1期6-10,共5页Journal of Logistics University of PAP（Medical Sciences）

基　　金：陕西省教育厅专项科研基金项目(2013JK0776)

摘　　要：【目的】探讨沉默信息调节因子1(silence information regulator 1,SIRT1)对高糖环境下血管内皮细胞活力及活性氧(reactive oxygen species,ROS)水平的影响。【方法】将人脐静脉血管内皮细胞分为正常组(5.5 mmol/L葡萄糖)、高糖组(30 mmol/L葡萄糖)、SRT组(30 mmol/L葡萄糖+SIRT1激活剂SRT)。噻唑蓝(methylthiazolyldiphenyl-tetrazolium bromide,MTT)检测细胞增殖,流式细胞术检测细胞凋亡,二氯二氢荧光素-乙酰乙酸酯法检测ROS水平,Western blotting检测SIRT1、p38丝裂原活化蛋白激酶(p38MAPK)、磷酸化的p38MAPK(p-p38MAPK)表达。【结果】高糖组、SRT组细胞存活率、SIRT1水平明显低于正常组,细胞凋亡率、ROS水平、p-p38MAPK水平明显高于正常组(P<0.05)。SRT组细胞存活率、SIRT1水平明显高于高糖组,细胞凋亡率、ROS水平、p-p38MAPK水平明显低于高糖组(P<0.05)。【结论】SIRT1能够拮抗高糖对血管内皮细胞活力抑制和凋亡促进作用,降低高糖环境下血管内皮细胞中ROS水平。【Objective】To explore the effects of silence information regulator 1（SIRT1） on the vascular endothelial cell viability and reactive oxygen species（ROS） level in high glucose environment.【Methods】The human umbilical vein endothelial cells were divided into normal group（5.5 mmol/L glucose）, high glucose group（30 mmol/L glucose）, SRT group（30 mmol/L glucose and SIRT1 activator SRT）. Cell proliferation was detected by MTT. Apoptosis was detected by flow cytometry. ROS levels were determined by DCFH-DA.The expressions of SIRT1, p38MAPK and p-p38MAPK were detected by Western blotting.【Results】The cell survival rates and SIRT1 levels in the high glucose group and SRT group were significantly lower than those in the normal group, while the apoptosis rates,ROS levels and p-p38MAPK levels were significantly higher than those in the normal group（P〈0.05）. The cell survival rate and SIRT1 level in the SRT group were significantly higher than those in the high glucose group, while the apoptosis rate, ROS level and pp38MAPK level were significantly lower than those in the high glucose group（P〈0.05）.【Conclusion】SIRT1 can antagonize the effect of high glucose on the viability inhibition and apoptosis promotion of vascular endothelial cells, and reduce ROS levels in vascular endothelial cells under high glucose environment.

关 键 词：血管内皮细胞 高糖 增殖活力 凋亡 沉默信息调节因子1 

分 类 号：R587.2[医药卫生—内分泌]