苦参碱体外抗念珠菌活性及对白色念珠菌细胞膜生物合成的影响  

Antifungal activity of matrinein vitro and its effect on membrane biosynthesis in Candida albicans

作　　者：李治建 周凡 窦勤 曹春雨[3] 斯拉甫.艾白 LI Zhijian;ZHOU Fan;DOU Qin;CAO Chunyu;Silafu· Aibai(Xinjiang Institute of Traditional Uighur Medicine,Xinjiang,Urumqi,830049,China;Xinjiang Hua Shidan Pharmaceutical Reaeareh CO.LTD,Xinjiang,Urumqi 830011,China;China Academy of Chinese Medical Science,Beijing 100700,China)

机构地区：[1]新疆维吾尔自治区维吾尔医药研究所,乌鲁木齐830049 [2]新疆华世丹药物研究有限责任公司,乌鲁木齐830011 [3]中国中医科学院中药研究所,北京100700

出　　处：《新疆医学》2018年第6期603-606,共4页Xinjiang Medical Journal

基　　金：乌鲁木齐市科学技术计划项目(Y151010027)

摘　　要：目的研究苦参碱对4种临床常见念珠菌的体外抗念珠菌活性,探讨其对白色念珠菌细胞膜生物合成的影响。方法采用美国临床实验室标准化协会(CLSI)的微量液基稀释法测定酵母菌药物敏感性方案(M27-A2),测定苦参碱对4株临床常见念珠菌的MIC值;采用高效液相色谱法(HPLC)法测定苦参碱对白色念珠菌细胞膜麦角固醇含量的影响,采用Elisa方法测定苦参碱对白色念珠菌细胞膜CYP51酶活性的影响。结果苦参碱对白色念珠菌(Candida albicans)MIC为6.25μg/mL,对近平滑念珠菌(Candida parapsilosis)的MIC为12.5μg/mL,对光滑念珠菌(Candida glabrata)的MIC为12.5μg/mL,对热带念珠菌(Candida tropicalis)的MIC为25.0μg/mL;苦参碱1.0-4.0μg/mL能使白色念珠菌细胞膜中的麦角固醇含量和CYP450酶活性下降,并有明显的剂量依赖性。结论苦参碱可通过抑制白色念珠菌细胞膜的CYP51酶活性,影响麦角固醇生物合成而发挥其抗真菌活性。Objective To study antifungal activity of matrinein vitro on 4 kinds of clinical common Candida and the effect on ergosterolbiosynthesis in the cell membranes of Candida albicans. Methods CLSI Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeastsmethod（M27-A2） was used to determine the susceptibilityof fungus（Candida albicans,Candidaparapsilosis, Candidaglabrata, Candida tropicalis）to matrine. 4 strainsC andida wereincubated for 48 h with different concentrations of matrineand fluconazole, and the content of ergosterol wasdetected by HPLC aftersaponifi cation and extraction. CYP51 activity in Trichophyton rubrum membrane was assayed using a commercial kit assay kit. Results The MIC（minimum inhibition concentrations）of matrineto Candida albicans, Candidaparapsilosis, Candidaglabrata, Candida tropicaliswas 6.25, 12.5, 12.5, 25.0 μg/mL, respectively.Matrine could decrease the content of ergosteroland the activity of CYP51 in Candida albicanswith a dose-dependent manner.Conclusion Matrinehad remarkable antifungal activity by inhibiting theergosterol biosynthesis in fungi.

关 键 词：苦参碱 白色念珠菌 麦角固醇 细胞膜 生物合成 

分 类 号：R96[医药卫生—药理学]