珠海市男男性行为人群HIV-1感染者基因突变分析  

Analysis of genetic mutation in HIV-1 infected MSM in Zhuhai

作　　者：林毅雄 魏泉德[1] 张丽荣[1] 京舒娉 周兰兰[1] 周毅[1] LIN Yi-xiong;WEI Quan-de;ZHANG Li-rong;JING Shu-ping;ZHOU Lan-lan;ZHOU Yi(Zhuhai Center for Disease Control and Prevention,Zhuhai,Guangdong 519000,China)

机构地区：[1]珠海市疾病预防控制中心,广东珠海519000

出　　处：《中国卫生检验杂志》2018年第15期1803-1805,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省医学科研基金项目(A2017491)

摘　　要：目的分析珠海市男男性行为(MSM)人群HIV-1流行亚型的分布与变化。方法收集珠海市2011年-2016年386例MSM人群HIV-1抗体阳性血清标本,提取病毒RNA,套式PCR对HIV-1 pol基因进行扩增、测序亚型序列分析。结果 CRF_07BC和CRF_01AE基因亚型占主要地位,分别为155例(占48.87%)和99例(占32.03%);其次是CRF55_01B亚型30例(占9.71%),同时也发现了B亚型11例(占3.56%)、CRF59_01B亚型10例(占3.24%)、CRF_08BC亚型3例(占0.97%)、CRF65_01B亚型3例(占0.97%)、CRF_01C亚型2例(占0.65%)。系统进化树显示CRF_07BC内部未见明显的分簇,CRF_01AE内部可分为两簇。结论珠海市MSM人群HIV-1基因亚型以CRF_01AE和CRF_07BC为主,其他多种基因型共同流行的现象,应加强该人群的干预和监测力度。Objective To investigate the distribution and changes of HIV-1 subtype prevalent in men who have sex with men（MSM） in Zhuhai. Methods Serum samples of positive HIV-1 from 386 MSM in Zhuhai were collected from 2011 to 2016.Virus RNA was extracted and nested PCR was used to amplify and sequence subtypes of HIV-1 pol gene. Results The highest proportions of the subtypes were CRF_07 BC（48. 87%,n = 155） and CRF_01 AE（32. 03%,n = 99）,followed by CRF55_01 B（9. 71%,n =30） and subtype B（3. 56%,n =11）,CRF59_01 B（3. 24%,n = 10）,CRF_08 BC（0. 97%,n = 3）,CRF65_01 B（0. 97%,n = 3）,CRF_01 C（0. 65%,n = 2）. The phylogenetic tree shows that there is no obvious clustering inside CRF_07 BC,and CRF_01 AE can be divided into two clusters. Conclusion CRF_07 BC and CRF_01 AE are the major epidemic HIV-1 infective strains among MSM in Zhuhai,and other recombinant subtypes are also prevalent. Intervention and monitoring should be enhanced among this MSM group.

关 键 词：人类免疫缺陷病毒 男男性行为 基因亚型 

分 类 号：R512.91[医药卫生—内科学]