Polo样激酶1(PLK1)的敲低及其对宫颈癌HeLa细胞生长的抑制作用  

Knockdown of Polo-like kinase 1 (PLK1) inhibits the growth of cervical cancer HeLa cells

作　　者：郭娜娜 高晨 陈蕾[1,2] GUO Nana;GAO Chen;CHEN Lei(Navy Clinical College,Anhui Medical University,Hefei 230032;Department of Obstetrics and Gynecology,Navy General Hospital of PLA,Beijing 100048;Hebei Normal University,Shijiazhuang 050024,China)

机构地区：[1]安徽医科大学海军临床学院,安徽合肥230032 [2]中国人民解放军海军总医院妇产科,北京100048 [3]河北师范大学,河北石家庄050024

出　　处：《细胞与分子免疫学杂志》2018年第4期334-340,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家高技术研究发展计划(863计划)(2014AA020708)

摘　　要：目的研究敲低Polo样激酶1(PLK1)后对宫颈癌HeLa细胞生长的作用。方法流式细胞术评测LAH4-L1载体对小干扰绿色荧光蛋白基因(siGFP)的递送效率;反转录PCR检测PLK1 mRNA水平,Western blot法检测PLK1蛋白水平;敲低PLK1水平后,采用CCK-8法检测HeLa细胞活性和生长抑制情况。结果 LAH4-L1-siPLK1纳米复合物可下调HeLa细胞PLK1约70%,并降低PLK1蛋白的表达,敲低PLK1后,可明显抑制HeLa细胞增殖。另外,LAH4-L1载体的基因递送效率能够稳定维持在较高水平。结论敲低HeLa细胞PLK1抑制癌细胞生长,LAH4-L1是一个较好的基因递送载体。Objective To investigate the effect of knockdown of Polo-like kinase 1（ PLK1） on the growth of HeLa cells.Methods Flow cytometry was used to evaluate the delivery efficiency of si GFP by LAH4-L1 vector. Reverse transcription PCR was used to detect the mRNA level of PLK1. Western blot analysis was performed to detect the level of PLK1 protein.And CCK-8 assay was used to detect the viability and growth of HeLa cells. Results About 70% gene and protein silence was achieved in HeLa cells after LAH4-L1-siPLK1 nanocomplexes transfection,and the proliferation of HeLa cells was significantly inhibited. Besides,the high delivery efficiency of LAH4-L1 could maintain at a stable level. Conclusion Knockdown of PLK1 in HeLa cells can inhibit the growth of HeLa cells. LAH4-L1 is a good gene delivery vector.

关 键 词：HELA细胞 宫颈癌 Polo样激酶1(PLK1) 基因沉默 LAH4-L1 

分 类 号：R392.33[医药卫生—免疫学] R737.33[医药卫生—基础医学]